Alternative Use of a Mini Exon of the L1 Gene Affects L1 Binding to Neural Ligands

Elena De Angelis, Thomas Brümmendorf, Ling Cheng, Vance Lemmon, Sue Kenwrick

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Neural cell adhesion molecule L1 is a cell surface glycoprotein required for the correct development of the nervous system. L1 exists as two isoforms encoded by mRNA species that either collectively incorporate or exclude exons 2 and 27. Neurons utilize only the full-length isoform, whereas Schwann cells, kidney cells, and blood lymphocytes only express the short form of L1. Still other cells, oligodendrocytes, regulate L1 isoform expression in a maturation-dependent manner. The RSLE motif encoded by exon 27 is known to have a role in clathrin-mediated endocytosis of L1, but the function of the exon 2-encoded motif (YEGHHV) is unknown. Here we show that this motif is required for the optimal binding of L1 to several neural ligands and is likely to be important for nervous system development. Thus, alternative use of exon 2 is a mechanism for regulating ligand interactions with L1.

Original languageEnglish
Pages (from-to)32738-32742
Number of pages5
JournalJournal of Biological Chemistry
Volume276
Issue number35
DOIs
StatePublished - Aug 31 2001
Externally publishedYes

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Exons
Genes
Ligands
Protein Isoforms
Neurology
Nervous System
Neural Cell Adhesion Molecule L1
RNA Isoforms
Clathrin
Lymphocytes
Schwann Cells
Membrane Glycoproteins
Oligodendroglia
Endocytosis
Neurons
Blood Cells
Blood
Cells
Kidney
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Alternative Use of a Mini Exon of the L1 Gene Affects L1 Binding to Neural Ligands. / De Angelis, Elena; Brümmendorf, Thomas; Cheng, Ling; Lemmon, Vance; Kenwrick, Sue.

In: Journal of Biological Chemistry, Vol. 276, No. 35, 31.08.2001, p. 32738-32742.

Research output: Contribution to journalArticle

De Angelis, Elena ; Brümmendorf, Thomas ; Cheng, Ling ; Lemmon, Vance ; Kenwrick, Sue. / Alternative Use of a Mini Exon of the L1 Gene Affects L1 Binding to Neural Ligands. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 35. pp. 32738-32742.
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