Abstract
Neural cell adhesion molecule L1 is a cell surface glycoprotein required for the correct development of the nervous system. L1 exists as two isoforms encoded by mRNA species that either collectively incorporate or exclude exons 2 and 27. Neurons utilize only the full-length isoform, whereas Schwann cells, kidney cells, and blood lymphocytes only express the short form of L1. Still other cells, oligodendrocytes, regulate L1 isoform expression in a maturation-dependent manner. The RSLE motif encoded by exon 27 is known to have a role in clathrin-mediated endocytosis of L1, but the function of the exon 2-encoded motif (YEGHHV) is unknown. Here we show that this motif is required for the optimal binding of L1 to several neural ligands and is likely to be important for nervous system development. Thus, alternative use of exon 2 is a mechanism for regulating ligand interactions with L1.
| Original language | English |
|---|---|
| Pages (from-to) | 32738-32742 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 276 |
| Issue number | 35 |
| DOIs | |
| State | Published - Aug 31 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Alternative Use of a Mini Exon of the L1 Gene Affects L1 Binding to Neural Ligands. / De Angelis, Elena; Brümmendorf, Thomas; Cheng, Ling; Lemmon, Vance; Kenwrick, Sue.
In: Journal of Biological Chemistry, Vol. 276, No. 35, 31.08.2001, p. 32738-32742.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Alternative Use of a Mini Exon of the L1 Gene Affects L1 Binding to Neural Ligands
AU - De Angelis, Elena
AU - Brümmendorf, Thomas
AU - Cheng, Ling
AU - Lemmon, Vance
AU - Kenwrick, Sue
PY - 2001/8/31
Y1 - 2001/8/31
N2 - Neural cell adhesion molecule L1 is a cell surface glycoprotein required for the correct development of the nervous system. L1 exists as two isoforms encoded by mRNA species that either collectively incorporate or exclude exons 2 and 27. Neurons utilize only the full-length isoform, whereas Schwann cells, kidney cells, and blood lymphocytes only express the short form of L1. Still other cells, oligodendrocytes, regulate L1 isoform expression in a maturation-dependent manner. The RSLE motif encoded by exon 27 is known to have a role in clathrin-mediated endocytosis of L1, but the function of the exon 2-encoded motif (YEGHHV) is unknown. Here we show that this motif is required for the optimal binding of L1 to several neural ligands and is likely to be important for nervous system development. Thus, alternative use of exon 2 is a mechanism for regulating ligand interactions with L1.
AB - Neural cell adhesion molecule L1 is a cell surface glycoprotein required for the correct development of the nervous system. L1 exists as two isoforms encoded by mRNA species that either collectively incorporate or exclude exons 2 and 27. Neurons utilize only the full-length isoform, whereas Schwann cells, kidney cells, and blood lymphocytes only express the short form of L1. Still other cells, oligodendrocytes, regulate L1 isoform expression in a maturation-dependent manner. The RSLE motif encoded by exon 27 is known to have a role in clathrin-mediated endocytosis of L1, but the function of the exon 2-encoded motif (YEGHHV) is unknown. Here we show that this motif is required for the optimal binding of L1 to several neural ligands and is likely to be important for nervous system development. Thus, alternative use of exon 2 is a mechanism for regulating ligand interactions with L1.
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U2 - 10.1074/jbc.M105156200
DO - 10.1074/jbc.M105156200
M3 - Article
VL - 276
SP - 32738
EP - 32742
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 35
ER -