Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1low mouse model of the disease

Anna Rita Migliaccio, Fabrizio Martelli, Maria Verrucci, Giovanni Migliaccio, Alessandro Maria Vannucchi, Hongyu Ni, Mingjiang Xu, Yi Jiang, Betty Nakamoto, Thalia Papayannopoulou, Ronald Hoffman

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Abstract

Objective: To assess whether alterations in the stromal cell-derived factor-1 (SDF-1)/CXCR4 occur in patients with primary myelofibrosis (PMF) and in Gata1low mice, an animal model for myelofibrosis, and whether these abnormalities might account for increased stem/progenitor cell trafficking. Materials and Methods: In the mouse, SDF-1 mRNA levels were assayed in liver, spleen, and marrow. SDF-1 protein levels were quantified in plasma and marrow and CXCR4 mRNA and protein levels were evaluated on stem/progenitor cells and megakaryocytes purified from the marrow. SDF-1 protein levels were also evaluated in plasma and in marrow biopsy specimens obtained from normal donors and PMF patients. Results: In Gata1low mice, the plasma SDF-1 protein was five times higher than normal in younger animals. Furthermore, SDF-1 immunostaining of marrow sections progressively increased with age. Similar abnormalities were observed in PMF patients. In fact, plasma SDF-1 levels in PMF patients were significantly higher (by twofold) than normal (p < 0.01) and SDF-1 immunostaining of marrow biopsy specimens demonstrated increased SDF-1 deposition in specific areas. In two of the patients, SDF-1 deposition was normalized by curative therapy with allogenic stem cell transplantation. Similar to what already has been reported for PMF patients, the marrow from Gata1low mice contained fewer CXCR4posCD117pos cells and these cells expressed low levels of CXCR4 mRNA and protein. Conclusion: Similar abnormalities in the SDF-1/CXCR4 axis are observed in PMF patients and in the Gata1low mice model of myelofibrosis. We suggest that these abnormalities contribute to the increased stem/progenitor cell trafficking observed in this mouse model as well as patients with PMF.

Original languageEnglish (US)
Pages (from-to)158-171
Number of pages14
JournalExperimental Hematology
Volume36
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

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Chemokine CXCL12
Primary Myelofibrosis
Bone Marrow
Stem Cells
Plasma Cells
Proteins
Messenger RNA
Biopsy
Megakaryocytes
Stem Cell Transplantation
Spleen
Animal Models
Tissue Donors

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Migliaccio, A. R., Martelli, F., Verrucci, M., Migliaccio, G., Vannucchi, A. M., Ni, H., ... Hoffman, R. (2008). Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1low mouse model of the disease. Experimental Hematology, 36(2), 158-171. https://doi.org/10.1016/j.exphem.2007.10.001

Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1low mouse model of the disease. / Migliaccio, Anna Rita; Martelli, Fabrizio; Verrucci, Maria; Migliaccio, Giovanni; Vannucchi, Alessandro Maria; Ni, Hongyu; Xu, Mingjiang; Jiang, Yi; Nakamoto, Betty; Papayannopoulou, Thalia; Hoffman, Ronald.

In: Experimental Hematology, Vol. 36, No. 2, 02.2008, p. 158-171.

Research output: Contribution to journalArticle

Migliaccio, AR, Martelli, F, Verrucci, M, Migliaccio, G, Vannucchi, AM, Ni, H, Xu, M, Jiang, Y, Nakamoto, B, Papayannopoulou, T & Hoffman, R 2008, 'Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1low mouse model of the disease', Experimental Hematology, vol. 36, no. 2, pp. 158-171. https://doi.org/10.1016/j.exphem.2007.10.001
Migliaccio, Anna Rita ; Martelli, Fabrizio ; Verrucci, Maria ; Migliaccio, Giovanni ; Vannucchi, Alessandro Maria ; Ni, Hongyu ; Xu, Mingjiang ; Jiang, Yi ; Nakamoto, Betty ; Papayannopoulou, Thalia ; Hoffman, Ronald. / Altered SDF-1/CXCR4 axis in patients with primary myelofibrosis and in the Gata1low mouse model of the disease. In: Experimental Hematology. 2008 ; Vol. 36, No. 2. pp. 158-171.
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abstract = "Objective: To assess whether alterations in the stromal cell-derived factor-1 (SDF-1)/CXCR4 occur in patients with primary myelofibrosis (PMF) and in Gata1low mice, an animal model for myelofibrosis, and whether these abnormalities might account for increased stem/progenitor cell trafficking. Materials and Methods: In the mouse, SDF-1 mRNA levels were assayed in liver, spleen, and marrow. SDF-1 protein levels were quantified in plasma and marrow and CXCR4 mRNA and protein levels were evaluated on stem/progenitor cells and megakaryocytes purified from the marrow. SDF-1 protein levels were also evaluated in plasma and in marrow biopsy specimens obtained from normal donors and PMF patients. Results: In Gata1low mice, the plasma SDF-1 protein was five times higher than normal in younger animals. Furthermore, SDF-1 immunostaining of marrow sections progressively increased with age. Similar abnormalities were observed in PMF patients. In fact, plasma SDF-1 levels in PMF patients were significantly higher (by twofold) than normal (p < 0.01) and SDF-1 immunostaining of marrow biopsy specimens demonstrated increased SDF-1 deposition in specific areas. In two of the patients, SDF-1 deposition was normalized by curative therapy with allogenic stem cell transplantation. Similar to what already has been reported for PMF patients, the marrow from Gata1low mice contained fewer CXCR4posCD117pos cells and these cells expressed low levels of CXCR4 mRNA and protein. Conclusion: Similar abnormalities in the SDF-1/CXCR4 axis are observed in PMF patients and in the Gata1low mice model of myelofibrosis. We suggest that these abnormalities contribute to the increased stem/progenitor cell trafficking observed in this mouse model as well as patients with PMF.",
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AU - Migliaccio, Giovanni

AU - Vannucchi, Alessandro Maria

AU - Ni, Hongyu

AU - Xu, Mingjiang

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AU - Papayannopoulou, Thalia

AU - Hoffman, Ronald

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