Abstract
IFN-Á is a crucial cytokine produced by T and NK cells. Previous work from our laboratory has reported that in T cells of BALB/c mice bearing the D1-DMBA-3 mammary tumor, IFN-Á production is down-regulated, due to decreased expression of IL-12 by macrophages of tumor bearers. IL-12 is the main inducer of IFN-Á production in T and NK cells. To exert its function, IL-12 interacts with its receptor (IL-12R), activating a JAK/STAT signaling pathway. Our investigation suggests that there is also a deficiency in the response to IL-12 by T cells from tumor hosts. The present work reports the results of RT-PCR experiments in the study of the IL-12R expression on T cells from normal and tumor bearers. Data showed a deficient expression of the IL-12R'2 chain on T cells from tumor hosts. Gene expression arrays on IL-12-activated T cells from normal and tumor bearers confirmed the RT-PCR results, and also showed decreased expression of IL-18R in tumor bearers' T cells. Arrays also showed down-regulated expression of JAK2, STAT 1, 3, 4 and IRF-1. Finally, increased expression of SOCS 1,3,4,5 and 7, as well of Protein inhibitor of activated STATs (Pias) 1 and y was also observed in tumor bearers' T cells.
| Original language | English |
|---|---|
| Pages (from-to) | 345-354 |
| Number of pages | 10 |
| Journal | Cancer Genomics and Proteomics |
| Volume | 1 |
| Issue number | 5-6 |
| State | Published - Sep 1 2004 |
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Keywords
- Gene expression microarrays
- IFN-Á
- IL-12 receptor
- Signal transduction
- T cells
- Tumor immunity
- Tumor-bearing mice
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Cancer Research
- Biochemistry
Cite this
Altered IL-12 signaling pathways contribute to the deficient IFN-A production by T splenocytes from tumor-bearing mice. / Torroella-Kouri, Marta; Herbert, Lynn M.; Perry, Giselle; Lopez, Diana M.
In: Cancer Genomics and Proteomics, Vol. 1, No. 5-6, 01.09.2004, p. 345-354.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Altered IL-12 signaling pathways contribute to the deficient IFN-A production by T splenocytes from tumor-bearing mice
AU - Torroella-Kouri, Marta
AU - Herbert, Lynn M.
AU - Perry, Giselle
AU - Lopez, Diana M
PY - 2004/9/1
Y1 - 2004/9/1
N2 - IFN-Á is a crucial cytokine produced by T and NK cells. Previous work from our laboratory has reported that in T cells of BALB/c mice bearing the D1-DMBA-3 mammary tumor, IFN-Á production is down-regulated, due to decreased expression of IL-12 by macrophages of tumor bearers. IL-12 is the main inducer of IFN-Á production in T and NK cells. To exert its function, IL-12 interacts with its receptor (IL-12R), activating a JAK/STAT signaling pathway. Our investigation suggests that there is also a deficiency in the response to IL-12 by T cells from tumor hosts. The present work reports the results of RT-PCR experiments in the study of the IL-12R expression on T cells from normal and tumor bearers. Data showed a deficient expression of the IL-12R'2 chain on T cells from tumor hosts. Gene expression arrays on IL-12-activated T cells from normal and tumor bearers confirmed the RT-PCR results, and also showed decreased expression of IL-18R in tumor bearers' T cells. Arrays also showed down-regulated expression of JAK2, STAT 1, 3, 4 and IRF-1. Finally, increased expression of SOCS 1,3,4,5 and 7, as well of Protein inhibitor of activated STATs (Pias) 1 and y was also observed in tumor bearers' T cells.
AB - IFN-Á is a crucial cytokine produced by T and NK cells. Previous work from our laboratory has reported that in T cells of BALB/c mice bearing the D1-DMBA-3 mammary tumor, IFN-Á production is down-regulated, due to decreased expression of IL-12 by macrophages of tumor bearers. IL-12 is the main inducer of IFN-Á production in T and NK cells. To exert its function, IL-12 interacts with its receptor (IL-12R), activating a JAK/STAT signaling pathway. Our investigation suggests that there is also a deficiency in the response to IL-12 by T cells from tumor hosts. The present work reports the results of RT-PCR experiments in the study of the IL-12R expression on T cells from normal and tumor bearers. Data showed a deficient expression of the IL-12R'2 chain on T cells from tumor hosts. Gene expression arrays on IL-12-activated T cells from normal and tumor bearers confirmed the RT-PCR results, and also showed decreased expression of IL-18R in tumor bearers' T cells. Arrays also showed down-regulated expression of JAK2, STAT 1, 3, 4 and IRF-1. Finally, increased expression of SOCS 1,3,4,5 and 7, as well of Protein inhibitor of activated STATs (Pias) 1 and y was also observed in tumor bearers' T cells.
KW - Gene expression microarrays
KW - IFN-Á
KW - IL-12 receptor
KW - Signal transduction
KW - T cells
KW - Tumor immunity
KW - Tumor-bearing mice
UR - http://www.scopus.com/inward/record.url?scp=66349126997&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66349126997&partnerID=8YFLogxK
M3 - Article
VL - 1
SP - 345
EP - 354
JO - Cancer Genomics and Proteomics
JF - Cancer Genomics and Proteomics
SN - 1109-6535
IS - 5-6
ER -