Altered hemolysin production in urine-grown uroisolates of Escherichia coli

R. Mittal, S. Sharma

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Fifteen uroisolates of E. coli were studies for both cell-free and cell-bound hemolysin production. Estimations were done in Trypticase soy broth (TSB, providing iron-replete medium) TSB +2,2′-bipyridine (providing experimentally created iron-depleted conditions) and pooled normal human urine (providing natural iron-depleted growth medium). In TSB 40% of strains showed no detectable cell-free hemolysin, they were able to produce it in the presence of 2,2′-bipyridine and more so when grown in urine. The cell-bound hemolysin was produced by all the strains in TSB, but in the presence of 2,2′-bipyridine and urine an insignificant increase was observed. All the strains when given 2nd and 3rd passage in urine, were found to elaborate significantly more cell-free as well as cell-bound hemolysin.

Original languageEnglish (US)
Pages (from-to)391-394
Number of pages4
JournalFolia Microbiologica
Volume36
Issue number4
DOIs
StatePublished - Aug 1991
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology

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