Altered configuration of Gc on the plasma membrane of transformed and malignant human B lymphocytes

Andre E. Nel, Manuel Navailles, David L. Emerson, Pascal Goldschmidt-Clermont, Satish K. Pathak, Kwong Y. Tsang, Robert M. Galbraith

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Normal human peripheral blood cells exhibit strong membrane fluorescence for Gc (vitamin D-binding protein), and this protein can form a close spatial relationship with integral membrane immunoglobulin (mIg) with evidence of codistribution in the lipid bilayer. In contrast, fluorescence for both Gc and mIg has been found in this study to be weak or absent in several B lymphoblastoid cell lines and in chronic lymphocytic leukemia B cells. Moreover, the comobility of these components, where detectable, was also impaired. In abnormal B cells, the intensity of membrane fluorescence for Gc was substantially increased after crosslinking of mIg with antibody, and the latter was also associated with increased specific radioiodination of Gc by lactoperoxidase. These results indicate that Gc can apparently become displaced under certain circumstances within or through the lipid bilayer. The altered content or membrane topography of Gc in such abnormal B cells might be associated with impaired expression and mobility of mIg.

Original languageEnglish (US)
Pages (from-to)191-202
Number of pages12
JournalClinical Immunology and Immunopathology
Volume37
Issue number2
DOIs
StatePublished - Nov 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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    Nel, A. E., Navailles, M., Emerson, D. L., Goldschmidt-Clermont, P., Pathak, S. K., Tsang, K. Y., & Galbraith, R. M. (1985). Altered configuration of Gc on the plasma membrane of transformed and malignant human B lymphocytes. Clinical Immunology and Immunopathology, 37(2), 191-202. https://doi.org/10.1016/0090-1229(85)90150-3