Altered behaviour following RNA interference knockdown of a C. elegans G-protein coupled receptor by ingested double stranded RNA

Ana Vaz Gomes, Claes Wahlestedt

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Using a systemic and continuous delivery method based on feeding on a particular strain of transformed Escherichia coli to induce double stranded RNA-mediated interference, we targeted the product of the npr-1 gene, a putative Caenorhabditis elegans homologue of a neuropeptide Y receptor, a G-protein coupled receptor. We were able to reproduce the social behaviour observed for the naturally occurring npr-1 mutant when wild type N2 Bristol eggs developed in a lawn of bacteria producing double stranded RNA for npr-1. This facile approach may also be useful when studying the function of other worm G-protein coupled receptors. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)R3-R5
JournalEuropean Journal of Pharmacology
Volume397
Issue number2-3
DOIs
StatePublished - Jun 2 2000
Externally publishedYes

Keywords

  • G-protein coupled receptor
  • npr-1
  • RNA-mediated interference

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

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