Altered angiotensin-converting enzyme in lung and extrapulmonary tissues of hypoxia-adapted rats

S. Oparil, A. J. Narkates, Robert Jackson, H. S. Ann

Research output: Contribution to journalArticle

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Abstract

The effects of exposing rats to hypoxia (10% O2) at normal atmospheric pressure for periods of 14 or 28 days on angiotensin-converting enzyme (ACE) activity and stores of angiotensin I (ANG I) and angiotensin II (ANG II) in lung, kidney, brain, and testis were examined. ACE activity was measured by spectrophotometric assay, and active sites of ACE were estimated by measuring the binding of 125I-351A [N-(1-carbonyl-3-phenyl-propyl)-L-lysyl-L-proline], a highly specific active site-directed inhibitor of ACE, to tissue homogenates and perfused lungs. Hypoxia exposure produced progressive reductions in ACE activity in lung homogenates and in ACE inhibitor binding to perfused lungs. ANG II levels in lungs from hypoxia-adapted animals were significantly less than air controls, suggesting that the reduction in intrapulmonary ACE activity was associated with reduced local generation of ANG II. ACE activity was increased in kidney and unchanged in brain and testis of hypoxia-adapted rats compared with air controls. Thus the effects of chronic hypoxia on catalytically active ACE and ACE active sites in the intact animal were organ specific. Adaptation to chronic hypoxia did not significantly alter plasma renin activity or ANG I or ANG II levels or serum ACE content. The hypoxia-induced alterations in lung and kidney ACE were reversible after return to a normoxic environment.

Original languageEnglish
Pages (from-to)218-227
Number of pages10
JournalJournal of Applied Physiology
Volume65
Issue number1
StatePublished - Jan 1 1988
Externally publishedYes

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Peptidyl-Dipeptidase A
Lung
Angiotensin II
Angiotensin I
Catalytic Domain
Kidney
Angiotensin-Converting Enzyme Inhibitors
Testis
Air
Hypoxia
Animal Structures
Brain Hypoxia
Atmospheric Pressure
Renin
Proline
Brain

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Altered angiotensin-converting enzyme in lung and extrapulmonary tissues of hypoxia-adapted rats. / Oparil, S.; Narkates, A. J.; Jackson, Robert; Ann, H. S.

In: Journal of Applied Physiology, Vol. 65, No. 1, 01.01.1988, p. 218-227.

Research output: Contribution to journalArticle

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