Alterations of purinergic regulation in mesenteric arteries of pannexin-1-knockout mice

O. O. Kiryukhina, D. K. Gaynullina, Y. V. Panchin, V. I. Shestopalov, O. S. Tarasova

Research output: Contribution to journalArticle

Abstract

Pannexin 1 (Panx!) forms plasma membrane channels that release ATP, an important vascular tone regulator. However, despite the abundant expression of Panx 1 in the vascular system, its effect on smooth muscle contraction is not evident in all arteries. In this study, we tested the hypothesis that the functional consequences of Panx I deficiency can be masked by the augmented action of ATP secreted in a Panxl- independent way. Experiments were performed on small mesenteric arteries obtained from Panxl- knockout mice (Panxl-/-) and C57B1/6 (Panxl+/+) male mice using wire myography of endothelium- denuded arterial preparations and reverse-Transcription quantitative PCR techniques. Arterial contractile responses to phenylephrine did not differ in two experimental groups. Ecto-ATPase inhibitor ARL67I56 (100 μM) potentiated the responses to phenylephrine in Panxl+/+ but not in Panxl-/-, while ARL67156 did not affect the contractile responses to the thromboxane A2 receptor agonist in any of the two groups. Contractile responses to exogenous ATP (10 pM) were smaller in Panxl+/+than in Panxl-/-. By contrast, NTPDasel mRNA content was higher in Panxl-/- than in PanxThese results suggest that ATP released from smooth muscle cells through Panx I channels can potentiate contractile responses of murine mesenteric arteries upon activation of a,-Adrenoceptors, in Panx I" mice an increased arterial ATP-sensitivity and diminished NTPDasel activity may augment the contractile effects of Panxl-independent ATP.

Original languageEnglish (US)
Pages (from-to)137-146
Number of pages10
JournalBiologicheskie Membrany
Volume34
Issue number6
DOIs
StatePublished - Jan 1 2017

Keywords

  • Ectonucleotidases
  • Pannexins
  • Thromboxane A2
  • α1-Adrenoceptors

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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