Alterations in the basement membrane zone in pili annulati hair follicles as demonstrated by electron microscopy and immunohistochemistry

K. A. Giehl, D. J P Ferguson, D. Dean, Y. H. Chuang, J. Allen, D. A R D E Berker, Antonella Tosti, R. P R Dawber, F. Wojnarowska

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Pili annulati is a rare autosomal dominant inherited hair shaft abnormality in which clinical examination reveals alternating light and dark bands leading to a shiny appearance of the hair. The clinically light bands are the abnormal areas due to cavities within the cortex. The pathogenesis remains unknown. Objectives: To investigate the expression of the basement membrane zone (BMZ) components in pili annulati hair follicles of the scalp. Methods: Transmission electron microscopy (TEM) was carried out on scalp sections of six individuals with pili annulati and six controls. Longitudinal sections of scalp tissues from four individuals with pili annulati and six normal controls were studied by immunohistochemistry with a panel of monoclonal antibodies to the following BMZ components: α6β4 integrin, laminin 5, LH39 antigen, laminin 1, collagen IV and collagen VII. Results: Using TEM, pili annulati scalp specimens exhibited a reduplicated lamina densa in the region of the root bulb in comparison with the single thin electron-dense band in controls. Using immunohistochemistry, there was a wavy BMZ in pili annulati follicles with antibodies to components of the lamina lucida, lamina densa and anchoring fibrils, whereas the BMZ in control hair follicles was as a smooth linear band. The expression of the hemidesmosome-associated α 6β4 integrin was linear in both pili annulati and control hair follicles. Conclusions: Our results suggest that the genetic defect may be a mutation in proteins involved in signalling and regulation of formation and degradation of the lamina densa and sublamina densa region resulting in abnormal assembly or remodelling of the BMZ.

Original languageEnglish
Pages (from-to)722-727
Number of pages6
JournalBritish Journal of Dermatology
Volume150
Issue number4
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

Fingerprint

Hair Follicle
Basement Membrane
Electron Microscopy
Immunohistochemistry
Scalp
Transmission Electron Microscopy
Integrins
Collagen
Hemidesmosomes
Pili annulati
Monoclonal Antibodies
Electrons
Light
Antigens
Mutation
Antibodies

Keywords

  • Basement membrane zone
  • Genodermatosis
  • Hair follicles
  • Immunohistochemistry
  • Pili annulati
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Dermatology

Cite this

Alterations in the basement membrane zone in pili annulati hair follicles as demonstrated by electron microscopy and immunohistochemistry. / Giehl, K. A.; Ferguson, D. J P; Dean, D.; Chuang, Y. H.; Allen, J.; Berker, D. A R D E; Tosti, Antonella; Dawber, R. P R; Wojnarowska, F.

In: British Journal of Dermatology, Vol. 150, No. 4, 01.04.2004, p. 722-727.

Research output: Contribution to journalArticle

Giehl, K. A. ; Ferguson, D. J P ; Dean, D. ; Chuang, Y. H. ; Allen, J. ; Berker, D. A R D E ; Tosti, Antonella ; Dawber, R. P R ; Wojnarowska, F. / Alterations in the basement membrane zone in pili annulati hair follicles as demonstrated by electron microscopy and immunohistochemistry. In: British Journal of Dermatology. 2004 ; Vol. 150, No. 4. pp. 722-727.
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abstract = "Background: Pili annulati is a rare autosomal dominant inherited hair shaft abnormality in which clinical examination reveals alternating light and dark bands leading to a shiny appearance of the hair. The clinically light bands are the abnormal areas due to cavities within the cortex. The pathogenesis remains unknown. Objectives: To investigate the expression of the basement membrane zone (BMZ) components in pili annulati hair follicles of the scalp. Methods: Transmission electron microscopy (TEM) was carried out on scalp sections of six individuals with pili annulati and six controls. Longitudinal sections of scalp tissues from four individuals with pili annulati and six normal controls were studied by immunohistochemistry with a panel of monoclonal antibodies to the following BMZ components: α6β4 integrin, laminin 5, LH39 antigen, laminin 1, collagen IV and collagen VII. Results: Using TEM, pili annulati scalp specimens exhibited a reduplicated lamina densa in the region of the root bulb in comparison with the single thin electron-dense band in controls. Using immunohistochemistry, there was a wavy BMZ in pili annulati follicles with antibodies to components of the lamina lucida, lamina densa and anchoring fibrils, whereas the BMZ in control hair follicles was as a smooth linear band. The expression of the hemidesmosome-associated α 6β4 integrin was linear in both pili annulati and control hair follicles. Conclusions: Our results suggest that the genetic defect may be a mutation in proteins involved in signalling and regulation of formation and degradation of the lamina densa and sublamina densa region resulting in abnormal assembly or remodelling of the BMZ.",
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T1 - Alterations in the basement membrane zone in pili annulati hair follicles as demonstrated by electron microscopy and immunohistochemistry

AU - Giehl, K. A.

AU - Ferguson, D. J P

AU - Dean, D.

AU - Chuang, Y. H.

AU - Allen, J.

AU - Berker, D. A R D E

AU - Tosti, Antonella

AU - Dawber, R. P R

AU - Wojnarowska, F.

PY - 2004/4/1

Y1 - 2004/4/1

N2 - Background: Pili annulati is a rare autosomal dominant inherited hair shaft abnormality in which clinical examination reveals alternating light and dark bands leading to a shiny appearance of the hair. The clinically light bands are the abnormal areas due to cavities within the cortex. The pathogenesis remains unknown. Objectives: To investigate the expression of the basement membrane zone (BMZ) components in pili annulati hair follicles of the scalp. Methods: Transmission electron microscopy (TEM) was carried out on scalp sections of six individuals with pili annulati and six controls. Longitudinal sections of scalp tissues from four individuals with pili annulati and six normal controls were studied by immunohistochemistry with a panel of monoclonal antibodies to the following BMZ components: α6β4 integrin, laminin 5, LH39 antigen, laminin 1, collagen IV and collagen VII. Results: Using TEM, pili annulati scalp specimens exhibited a reduplicated lamina densa in the region of the root bulb in comparison with the single thin electron-dense band in controls. Using immunohistochemistry, there was a wavy BMZ in pili annulati follicles with antibodies to components of the lamina lucida, lamina densa and anchoring fibrils, whereas the BMZ in control hair follicles was as a smooth linear band. The expression of the hemidesmosome-associated α 6β4 integrin was linear in both pili annulati and control hair follicles. Conclusions: Our results suggest that the genetic defect may be a mutation in proteins involved in signalling and regulation of formation and degradation of the lamina densa and sublamina densa region resulting in abnormal assembly or remodelling of the BMZ.

AB - Background: Pili annulati is a rare autosomal dominant inherited hair shaft abnormality in which clinical examination reveals alternating light and dark bands leading to a shiny appearance of the hair. The clinically light bands are the abnormal areas due to cavities within the cortex. The pathogenesis remains unknown. Objectives: To investigate the expression of the basement membrane zone (BMZ) components in pili annulati hair follicles of the scalp. Methods: Transmission electron microscopy (TEM) was carried out on scalp sections of six individuals with pili annulati and six controls. Longitudinal sections of scalp tissues from four individuals with pili annulati and six normal controls were studied by immunohistochemistry with a panel of monoclonal antibodies to the following BMZ components: α6β4 integrin, laminin 5, LH39 antigen, laminin 1, collagen IV and collagen VII. Results: Using TEM, pili annulati scalp specimens exhibited a reduplicated lamina densa in the region of the root bulb in comparison with the single thin electron-dense band in controls. Using immunohistochemistry, there was a wavy BMZ in pili annulati follicles with antibodies to components of the lamina lucida, lamina densa and anchoring fibrils, whereas the BMZ in control hair follicles was as a smooth linear band. The expression of the hemidesmosome-associated α 6β4 integrin was linear in both pili annulati and control hair follicles. Conclusions: Our results suggest that the genetic defect may be a mutation in proteins involved in signalling and regulation of formation and degradation of the lamina densa and sublamina densa region resulting in abnormal assembly or remodelling of the BMZ.

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KW - Genodermatosis

KW - Hair follicles

KW - Immunohistochemistry

KW - Pili annulati

KW - Transmission electron microscopy

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