Alterations in phosphoinositide metabolism associated with 17β-estradiol and growth factor treatment of MCF-7 breast cancer cells

C. E. Freter, Marc E Lippman, A. Cheville, S. Zinn, E. P. Gelmann

Research output: Contribution to journalArticle

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Abstract

Steady-state levels of phosphatidyl inositol (PtdIns) turnover are examined in MCF-7 human breast cancer cells in response to estradiol treatment. Elevated levels of PtdIns are observed 12-24 hr after estradiol treatment, occur at estradiol concentrations as low as 10-12 M, and are competitively blocked by the antiestrogen LY117018. MCF-7 cells secrete a transforming growth factor (TGF) α-like material which can partly replace estradiol in conferring tumorgenicity in nude mice. We show that acute or chronic treatment of MCF-7 cells with TGFα results in elevated PtdIns turnover and that chronic treatment increases growth rate. In contrast TGFβ is growth inhibitory and blocks estradiol-induced increases in PtdIns turnover. A phosphatidyl inositol 4,5-bisphosphate specific phospolipase-C activity has been identified and is elevated in association with estradiol treatment. These data are consistent with estradiol-induced autocrine growth factors, including TGFα, acting through the PtdIns turnover pathway as part of their mechanism of action.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalMolecular Endocrinology
Volume2
Issue number2
StatePublished - Jan 1 1988
Externally publishedYes

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Phosphatidylinositols
Estradiol
Intercellular Signaling Peptides and Proteins
Breast Neoplasms
Transforming Growth Factors
MCF-7 Cells
LY 117018
Estrogen Receptor Modulators
Growth
Nude Mice

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Alterations in phosphoinositide metabolism associated with 17β-estradiol and growth factor treatment of MCF-7 breast cancer cells. / Freter, C. E.; Lippman, Marc E; Cheville, A.; Zinn, S.; Gelmann, E. P.

In: Molecular Endocrinology, Vol. 2, No. 2, 01.01.1988, p. 159-166.

Research output: Contribution to journalArticle

Freter, C. E. ; Lippman, Marc E ; Cheville, A. ; Zinn, S. ; Gelmann, E. P. / Alterations in phosphoinositide metabolism associated with 17β-estradiol and growth factor treatment of MCF-7 breast cancer cells. In: Molecular Endocrinology. 1988 ; Vol. 2, No. 2. pp. 159-166.
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