Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients

Erica Leonor Hofer, Vincent La Russa, Alba Elizabeth Honegger, Eduardo Oscar Bullorsky, Raúl Horacio Bordenave, Norma Alejandra Chasseing

Research output: Contribution to journalArticle

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Abstract

Previously, we reported a deficient cloning capacity of the bone marrow (BM) mesenchymal stem cells to give colony-forming unit fibroblast (CFU-F) and an inefficient confluence capacity of BM stromal cells in advanced untreated lung cancer patients (LCP) and breast cancer patients (BCP). Moreover, a decreased level of bFGF at day 7 in the conditioned media from BM CFU-F cultures was found in both cancer groups when compared to the normal range. The current study was specially undertaken, to evaluate the percentage of subconfluent fibroblasts expressing receptors (R) of interleukin-1 (IL-1), platelet-derived growth factor (PDGF), fibroblast growth factor (FGF), transforming growth factor (TGF-β), epidermal growth factor (EGF), and the proteins c-Fos and c-Myc in BM primary cultures from untreated LCP and BCP. An immunocytochemical study on subconfluent BM fibroblast cultures from 13 healthy patients, 16 LCP, and 8 BCP was performed, using as primary antibodies, anti-type I of IL-1 R (IL-1R-1), anti-α, β chains of PDGF R (PDGFR-α, PDGFR-β), anti-type I of FGF R (FGFR-I), anti-type I, II, and III of TGF-β R (TGF-βR-I, TGF-βR-II, and TGF-βR-III), anti-EGF R, anti-c-Fos, and anti-c-Myc. A diminished percentage of subconfluent fibroblasts expressing PDGFR-α, TGFβR-I, II, III, EGFR, and FGFR-I was found in LCP and BCP compared to healthy patients. A diminished percentage of subconfluent fibroblasts expressing c-Fos and c-Myc was found in patients when compared to healthy patients. The alterations we describe could help to explain the deficiency regarding the proliferative and confluence capacity of BM stroma cells in cancer patients.

Original languageEnglish
Pages (from-to)587-594
Number of pages8
JournalStem Cells and Development
Volume14
Issue number5
DOIs
StatePublished - Oct 1 2005

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Proto-Oncogene Proteins c-myc
Fibroblast Growth Factor Receptors
Platelet-Derived Growth Factor
Transforming Growth Factors
Interleukin-1
Epidermal Growth Factor
Lung Neoplasms
Bone Marrow
Breast Neoplasms
Fibroblasts
Fibroblast Growth Factors
Mesenchymal Stromal Cells
Stem Cells
Proto-Oncogene Proteins c-fos
Interleukin-1 Receptors
Conditioned Culture Medium
Bone Marrow Cells

ASJC Scopus subject areas

  • Hematology

Cite this

Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients. / Hofer, Erica Leonor; La Russa, Vincent; Honegger, Alba Elizabeth; Bullorsky, Eduardo Oscar; Bordenave, Raúl Horacio; Chasseing, Norma Alejandra.

In: Stem Cells and Development, Vol. 14, No. 5, 01.10.2005, p. 587-594.

Research output: Contribution to journalArticle

Hofer, Erica Leonor ; La Russa, Vincent ; Honegger, Alba Elizabeth ; Bullorsky, Eduardo Oscar ; Bordenave, Raúl Horacio ; Chasseing, Norma Alejandra. / Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients. In: Stem Cells and Development. 2005 ; Vol. 14, No. 5. pp. 587-594.
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T1 - Alteration on the expression of IL-1, PDGF, TGF-β, EGF, and FGF receptors and c-Fos and c-Myc proteins in bone marrow mesenchymal stroma cells from advanced untreated lung and breast cancer patients

AU - Hofer, Erica Leonor

AU - La Russa, Vincent

AU - Honegger, Alba Elizabeth

AU - Bullorsky, Eduardo Oscar

AU - Bordenave, Raúl Horacio

AU - Chasseing, Norma Alejandra

PY - 2005/10/1

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