TY - JOUR
T1 - Alteration of protein metabolism in individual, identified neurons from Aplysia
AU - Wilson, David L.
PY - 1976/9
Y1 - 1976/9
N2 - A search for control mechanisms governing protein metabolism in neurons from Aplysia californica has uncovered two examples of altered patterns of newly synthesized proteins. The pattern of newly synthesized proteins in the R2 neuron is altered when protein synthesis occurs at elevated temperatures (22-30°C as compared with 13-15°C). The processing of newly synthesized 12,000 dalton (12k) material to 6-9,000 dalton (6-9k) size in the R15 neuron can be blocked by certain ion replacements. If acetate replaces chloride in the incubation medium during the synthesis of 12k material, an early step in the processing, prior to the actual breakdown of 12k material, is blocked. Experiments with RNA synthesis inhibitors indicate that none of the mRNAs which code for abundantly synthesized protein species in the R2 or R15 neurons have short (less than 4 hr) half lives. This result has implications for an earlier report of regulation of protein synthesis in the R15 neuron.
AB - A search for control mechanisms governing protein metabolism in neurons from Aplysia californica has uncovered two examples of altered patterns of newly synthesized proteins. The pattern of newly synthesized proteins in the R2 neuron is altered when protein synthesis occurs at elevated temperatures (22-30°C as compared with 13-15°C). The processing of newly synthesized 12,000 dalton (12k) material to 6-9,000 dalton (6-9k) size in the R15 neuron can be blocked by certain ion replacements. If acetate replaces chloride in the incubation medium during the synthesis of 12k material, an early step in the processing, prior to the actual breakdown of 12k material, is blocked. Experiments with RNA synthesis inhibitors indicate that none of the mRNAs which code for abundantly synthesized protein species in the R2 or R15 neurons have short (less than 4 hr) half lives. This result has implications for an earlier report of regulation of protein synthesis in the R15 neuron.
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U2 - 10.1002/neu.480070504
DO - 10.1002/neu.480070504
M3 - Article
C2 - 978202
AN - SCOPUS:0017132798
VL - 7
SP - 407
EP - 416
JO - Developmental Neurobiology
JF - Developmental Neurobiology
SN - 0022-3034
IS - 5
ER -