Alteration of protein metabolism in individual, identified neurons from Aplysia

David L. Wilson

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A search for control mechanisms governing protein metabolism in neurons from Aplysia californica has uncovered two examples of altered patterns of newly synthesized proteins. The pattern of newly synthesized proteins in the R2 neuron is altered when protein synthesis occurs at elevated temperatures (22-30°C as compared with 13-15°C). The processing of newly synthesized 12,000 dalton (12k) material to 6-9,000 dalton (6-9k) size in the R15 neuron can be blocked by certain ion replacements. If acetate replaces chloride in the incubation medium during the synthesis of 12k material, an early step in the processing, prior to the actual breakdown of 12k material, is blocked. Experiments with RNA synthesis inhibitors indicate that none of the mRNAs which code for abundantly synthesized protein species in the R2 or R15 neurons have short (less than 4 hr) half lives. This result has implications for an earlier report of regulation of protein synthesis in the R15 neuron.

Original languageEnglish (US)
Pages (from-to)407-416
Number of pages10
JournalJournal of Neurobiology
Volume7
Issue number5
DOIs
StatePublished - Sep 1976

ASJC Scopus subject areas

  • Neuroscience(all)
  • Cellular and Molecular Neuroscience

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