Allogeneic bone marrow supports human islet β cell survival and function over six months

LuGuang Luo, Evangelos V Badiavas, John ZQ Luo, Abby Maizel

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this study, we have established a new strategy increasing human islet longevity utilizing allogeneic whole bone marrow (BM) co-cultured with human islets. The cultured islets' function and survival have been evaluated by analysis of insulin secretion in response to high-glucose-challenge, morphological evaluation of cell growth. Human islet only culture failed to reveal evidence of long term survival, growth or function in terms of insulin release or insulin response to glucose challenge. These results indicate that BM increases islet survival and function with the eventual formation of pancreatic endocrine tissue capable of sustaining β cell fuction.

Original languageEnglish
Pages (from-to)859-864
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume361
Issue number4
DOIs
StatePublished - Oct 5 2007
Externally publishedYes

Fingerprint

Islets of Langerhans
Cell Survival
Bone
Bone Marrow
Cells
Insulin
Survival
Glucose
Cell growth
Growth
Cell culture
Tissue

Keywords

  • Allogeneic bone marrow
  • Diabetes
  • Function
  • Growth
  • Human islet
  • Insulin
  • Survival

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Allogeneic bone marrow supports human islet β cell survival and function over six months. / Luo, LuGuang; Badiavas, Evangelos V; Luo, John ZQ; Maizel, Abby.

In: Biochemical and Biophysical Research Communications, Vol. 361, No. 4, 05.10.2007, p. 859-864.

Research output: Contribution to journalArticle

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