Alloantigens determined by a second D region locus elicit a strong in vitro cytotoxic response

T. H. Hansen, R. B. Levy

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The newly defined L antigen (previously designated D') has been studied with the CML assay. BALB/c-H-2(db) mice, which carry a loss mutation in the D region, were found to generate anti-L cytotoxic effector cells when stimulated with wild-type (BALB/c) cells. When the target cells were treated with an antiserum against L, cytolysis by BALB/c-H-2(db) anti-BALB/c effectors was completely blocked. However, an antiserum against D, the antigen that bears the D region private specificity, had no effect on the anti-L cytotoxic response. In a control CML experiment that was specific for the D antigen, the antiserum to L had no effect, whereas the antiserum to D blocked completely. These results indicate that the L and D antigens are recognized in a CML response as separate entities on the cell surface. In an allogeneic response to the products of the D region, cytotoxic effectors were generated that were specific for L in addition to those specific for D. This conclusion was supported by blocking experiments with either antisera or unlabeled targets. These functional studies thus extend previous co-capping and immunochemical studies, and further support the hypothesis that L is a third transplantation antigen similar to the K and D antigens.

Original languageEnglish (US)
Pages (from-to)1836-1840
Number of pages5
JournalJournal of Immunology
Volume120
Issue number6
StatePublished - Dec 1 1978
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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