Abstract
To investigate the possible relationship between allergy and otitis media with effusion (OME), we investigated the hypothesis that allergen presentation to the middle ear causes functional disruption of the eustachian tube predisposing to the development of OME. Thirteen of 19 Brown-Norway rats were sensitized to ovalbumin, and the remaining 6 served as nonallergic controls. To mimic subclinical exposure to allergen, we transtympanically injected ovalbumin at a dose (0.01 mg) that produced no changes detectable by otologic examination. Next, both allergic and nonallergic rats were exposed to transtympanic injection of either low-dose (10 μg/mL) or high-dose (100 μg/mL) lipopolysaccharide to simulate bacterial exposure. The allergic rats were found to have larger middle ear effusions when exposed to high-dose lipopolysaccharide as compared with the nonallergic controls. This response could be inhibited by diphenhydramine. We conclude that allergen presentation to the middle ear of allergic rats causes eustachian tube dysfunction predisposing to OME.
| Original language | English |
|---|---|
| Pages (from-to) | 687-692 |
| Number of pages | 6 |
| Journal | Otolaryngology - Head and Neck Surgery |
| Volume | 121 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 23 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Otorhinolaryngology
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Allergy increases susceptibility to otitis media with effusion in a rat model. / Labadie, Robert F.; Jewett, Brian S.; Hart, Craig F.; Prazma, Jiri; Pillsbury, Harold C.
In: Otolaryngology - Head and Neck Surgery, Vol. 121, No. 6, 23.12.1999, p. 687-692.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Allergy increases susceptibility to otitis media with effusion in a rat model
AU - Labadie, Robert F.
AU - Jewett, Brian S.
AU - Hart, Craig F.
AU - Prazma, Jiri
AU - Pillsbury, Harold C.
PY - 1999/12/23
Y1 - 1999/12/23
N2 - To investigate the possible relationship between allergy and otitis media with effusion (OME), we investigated the hypothesis that allergen presentation to the middle ear causes functional disruption of the eustachian tube predisposing to the development of OME. Thirteen of 19 Brown-Norway rats were sensitized to ovalbumin, and the remaining 6 served as nonallergic controls. To mimic subclinical exposure to allergen, we transtympanically injected ovalbumin at a dose (0.01 mg) that produced no changes detectable by otologic examination. Next, both allergic and nonallergic rats were exposed to transtympanic injection of either low-dose (10 μg/mL) or high-dose (100 μg/mL) lipopolysaccharide to simulate bacterial exposure. The allergic rats were found to have larger middle ear effusions when exposed to high-dose lipopolysaccharide as compared with the nonallergic controls. This response could be inhibited by diphenhydramine. We conclude that allergen presentation to the middle ear of allergic rats causes eustachian tube dysfunction predisposing to OME.
AB - To investigate the possible relationship between allergy and otitis media with effusion (OME), we investigated the hypothesis that allergen presentation to the middle ear causes functional disruption of the eustachian tube predisposing to the development of OME. Thirteen of 19 Brown-Norway rats were sensitized to ovalbumin, and the remaining 6 served as nonallergic controls. To mimic subclinical exposure to allergen, we transtympanically injected ovalbumin at a dose (0.01 mg) that produced no changes detectable by otologic examination. Next, both allergic and nonallergic rats were exposed to transtympanic injection of either low-dose (10 μg/mL) or high-dose (100 μg/mL) lipopolysaccharide to simulate bacterial exposure. The allergic rats were found to have larger middle ear effusions when exposed to high-dose lipopolysaccharide as compared with the nonallergic controls. This response could be inhibited by diphenhydramine. We conclude that allergen presentation to the middle ear of allergic rats causes eustachian tube dysfunction predisposing to OME.
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U2 - 10.1053/hn.1999.v121.a100502
DO - 10.1053/hn.1999.v121.a100502
M3 - Article
VL - 121
SP - 687
EP - 692
JO - Otolaryngology - Head and Neck Surgery (United States)
JF - Otolaryngology - Head and Neck Surgery (United States)
SN - 0194-5998
IS - 6
ER -