All CVB serotypes and clinical isolates induce irreversible cytopathic effects in primary cardiomyocytes

Jeonghyun Ahn, Chul Hyun Joo, Ilsun Seo, Dong Hou Kim, Yoo Kyum Kim, Heuiran Lee

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Coxsackievirus B3 (CVB3) has been identified as a major causative agent of acute and chronic myocarditis, but the involvement of other CVB serotypes in myocarditis has not been investigated. To dissect the pathological properties of different CVB serotypes toward primary cardiomyocytes, we tested their effects on primary cardiomyocyte cultures from neonatal rats. Morphological abnormalities were examined by both light and fluorescence microscopy after Hoechst 33342 staining, and loss of cell viability was estimated by MTT assay. All six CVB serotypes showed a similar degree of severe toxicity toward primary cardiomyocytes. CVB clinical isolates had cytopathic effects (CPEs) similar to those of their respective CVB reference strains. Within 1-2 days of infection with multiplicities of infection MOI 50, the cells began to experience morphological changes including cell shrinkage, rounding-up, and slight nuclear condensation. The irreversible loss of cell viability was readily observed within 3-5 days following virus infection. These results suggest that all six CVB serotypes induce direct, irreversible toxicity towards cardiomyocytes, which eventually leads to the death of infected cells. These findings indicate that the variations in CVB serotype are not the limiting factor determining the susceptibility of cardiomyocytes to CVB infection.

Original languageEnglish (US)
Pages (from-to)290-294
Number of pages5
JournalJournal of Medical Virology
Volume75
Issue number2
DOIs
StatePublished - Feb 1 2005
Externally publishedYes

Fingerprint

Cardiac Myocytes
Myocarditis
Cell Survival
Infection
Enterovirus
Virus Diseases
Fluorescence Microscopy
Cell Death
Serogroup
Staining and Labeling
Light

Keywords

  • Cardiomyocyte
  • Coxsackievirus B
  • Cytopathic effect
  • MTT assay

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

All CVB serotypes and clinical isolates induce irreversible cytopathic effects in primary cardiomyocytes. / Ahn, Jeonghyun; Joo, Chul Hyun; Seo, Ilsun; Kim, Dong Hou; Kim, Yoo Kyum; Lee, Heuiran.

In: Journal of Medical Virology, Vol. 75, No. 2, 01.02.2005, p. 290-294.

Research output: Contribution to journalArticle

Ahn, Jeonghyun ; Joo, Chul Hyun ; Seo, Ilsun ; Kim, Dong Hou ; Kim, Yoo Kyum ; Lee, Heuiran. / All CVB serotypes and clinical isolates induce irreversible cytopathic effects in primary cardiomyocytes. In: Journal of Medical Virology. 2005 ; Vol. 75, No. 2. pp. 290-294.
@article{ef58810086be4388ab7968190b3fcc2b,
title = "All CVB serotypes and clinical isolates induce irreversible cytopathic effects in primary cardiomyocytes",
abstract = "Coxsackievirus B3 (CVB3) has been identified as a major causative agent of acute and chronic myocarditis, but the involvement of other CVB serotypes in myocarditis has not been investigated. To dissect the pathological properties of different CVB serotypes toward primary cardiomyocytes, we tested their effects on primary cardiomyocyte cultures from neonatal rats. Morphological abnormalities were examined by both light and fluorescence microscopy after Hoechst 33342 staining, and loss of cell viability was estimated by MTT assay. All six CVB serotypes showed a similar degree of severe toxicity toward primary cardiomyocytes. CVB clinical isolates had cytopathic effects (CPEs) similar to those of their respective CVB reference strains. Within 1-2 days of infection with multiplicities of infection MOI 50, the cells began to experience morphological changes including cell shrinkage, rounding-up, and slight nuclear condensation. The irreversible loss of cell viability was readily observed within 3-5 days following virus infection. These results suggest that all six CVB serotypes induce direct, irreversible toxicity towards cardiomyocytes, which eventually leads to the death of infected cells. These findings indicate that the variations in CVB serotype are not the limiting factor determining the susceptibility of cardiomyocytes to CVB infection.",
keywords = "Cardiomyocyte, Coxsackievirus B, Cytopathic effect, MTT assay",
author = "Jeonghyun Ahn and Joo, {Chul Hyun} and Ilsun Seo and Kim, {Dong Hou} and Kim, {Yoo Kyum} and Heuiran Lee",
year = "2005",
month = "2",
day = "1",
doi = "10.1002/jmv.20269",
language = "English (US)",
volume = "75",
pages = "290--294",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - All CVB serotypes and clinical isolates induce irreversible cytopathic effects in primary cardiomyocytes

AU - Ahn, Jeonghyun

AU - Joo, Chul Hyun

AU - Seo, Ilsun

AU - Kim, Dong Hou

AU - Kim, Yoo Kyum

AU - Lee, Heuiran

PY - 2005/2/1

Y1 - 2005/2/1

N2 - Coxsackievirus B3 (CVB3) has been identified as a major causative agent of acute and chronic myocarditis, but the involvement of other CVB serotypes in myocarditis has not been investigated. To dissect the pathological properties of different CVB serotypes toward primary cardiomyocytes, we tested their effects on primary cardiomyocyte cultures from neonatal rats. Morphological abnormalities were examined by both light and fluorescence microscopy after Hoechst 33342 staining, and loss of cell viability was estimated by MTT assay. All six CVB serotypes showed a similar degree of severe toxicity toward primary cardiomyocytes. CVB clinical isolates had cytopathic effects (CPEs) similar to those of their respective CVB reference strains. Within 1-2 days of infection with multiplicities of infection MOI 50, the cells began to experience morphological changes including cell shrinkage, rounding-up, and slight nuclear condensation. The irreversible loss of cell viability was readily observed within 3-5 days following virus infection. These results suggest that all six CVB serotypes induce direct, irreversible toxicity towards cardiomyocytes, which eventually leads to the death of infected cells. These findings indicate that the variations in CVB serotype are not the limiting factor determining the susceptibility of cardiomyocytes to CVB infection.

AB - Coxsackievirus B3 (CVB3) has been identified as a major causative agent of acute and chronic myocarditis, but the involvement of other CVB serotypes in myocarditis has not been investigated. To dissect the pathological properties of different CVB serotypes toward primary cardiomyocytes, we tested their effects on primary cardiomyocyte cultures from neonatal rats. Morphological abnormalities were examined by both light and fluorescence microscopy after Hoechst 33342 staining, and loss of cell viability was estimated by MTT assay. All six CVB serotypes showed a similar degree of severe toxicity toward primary cardiomyocytes. CVB clinical isolates had cytopathic effects (CPEs) similar to those of their respective CVB reference strains. Within 1-2 days of infection with multiplicities of infection MOI 50, the cells began to experience morphological changes including cell shrinkage, rounding-up, and slight nuclear condensation. The irreversible loss of cell viability was readily observed within 3-5 days following virus infection. These results suggest that all six CVB serotypes induce direct, irreversible toxicity towards cardiomyocytes, which eventually leads to the death of infected cells. These findings indicate that the variations in CVB serotype are not the limiting factor determining the susceptibility of cardiomyocytes to CVB infection.

KW - Cardiomyocyte

KW - Coxsackievirus B

KW - Cytopathic effect

KW - MTT assay

UR - http://www.scopus.com/inward/record.url?scp=11144237443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144237443&partnerID=8YFLogxK

U2 - 10.1002/jmv.20269

DO - 10.1002/jmv.20269

M3 - Article

C2 - 15602738

AN - SCOPUS:11144237443

VL - 75

SP - 290

EP - 294

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 2

ER -