Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination in vitro

Siliang Wu, Ming Shuo Chen, Patrice Maurel, Yee Shuan Lee, Mary B Bunge, Treena Livingston Arinzeh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective. Polyvinylidene fluoride-trifluoroethylene (PVDF-TrFE), which is a piezoelectric, biocompatible polymer, holds promise as a scaffold in combination with Schwann cells (SCs) for spinal cord repair. Piezoelectric materials can generate electrical activity in response to mechanical deformation, which could potentially stimulate spinal cord axon regeneration. Our goal in this study was to investigate PVDF-TrFE scaffolds consisting of aligned fibers in supporting SC growth and SC-supported neurite extension and myelination in vitro. Approach. Aligned fibers of PVDF-TrFE were fabricated using the electrospinning technique. SCs and dorsal root ganglion (DRG) explants were co-cultured to evaluate SC-supported neurite extension and myelination on PVDF-TrFE scaffolds. Main results. PVDF-TrFE scaffolds supported SC growth and neurite extension, which was further enhanced by coating the scaffolds with Matrigel. SCs were oriented and neurites extended along the length of the aligned fibers. SCs in co-culture with DRGs on PVDF-TrFE scaffolds promoted longer neurite extension as compared to scaffolds without SCs. In addition to promoting neurite extension, SCs also formed myelin around DRG neurites on PVDF-TrFE scaffolds. Significance. This study demonstrated PVDF-TrFE scaffolds containing aligned fibers supported SC-neurite extension and myelination. The combination of SCs and PVDF-TrFE scaffolds may be a promising tissue engineering strategy for spinal cord repair.

Original languageEnglish (US)
Article number056010
JournalJournal of Neural Engineering
Volume15
Issue number5
DOIs
StatePublished - Jul 18 2018

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Schwann Cells
Neurites
Scaffolds
Cells
Scaffolds (biology)
Fibers
Spinal Cord Regeneration
Cell growth
Repair
Piezoelectric materials
Electrospinning
Spinal Ganglia
Tissue engineering
trifluoroethene
In Vitro Techniques
polyvinylidene fluoride
Coatings
Diagnosis-Related Groups
Polymers
Tissue Engineering

Keywords

  • electrospinning
  • myelination
  • neural tissue engineering
  • neurite extension
  • PVDF-TrFE
  • Schwann cell

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

Cite this

Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination in vitro. / Wu, Siliang; Chen, Ming Shuo; Maurel, Patrice; Lee, Yee Shuan; Bunge, Mary B; Arinzeh, Treena Livingston.

In: Journal of Neural Engineering, Vol. 15, No. 5, 056010, 18.07.2018.

Research output: Contribution to journalArticle

Wu, Siliang ; Chen, Ming Shuo ; Maurel, Patrice ; Lee, Yee Shuan ; Bunge, Mary B ; Arinzeh, Treena Livingston. / Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination in vitro. In: Journal of Neural Engineering. 2018 ; Vol. 15, No. 5.
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