ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model

Giovanni Ambrosino, S. Varotto, S. Basso, D. Galavotti, A. Cecchetto, P. Carraro, A. Naso, G. De Silvestro, M. Plebani, G. Giron, G. Abatangelo, D. Donato, G. P. Braga, A. Cestrone, L. Marrelli, M. Trombetta, V. Lorenzelli, A. Picardi, M. L. Valente, G. PaluA. Colantoni, D. Van Thiel, C. Ricordi, D. F. D'Amico

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Long-term maintenance of viability and expression of differentiated hepatocyte function is crucial for bioartificial liver support. We developed a new bioreactor design (ALEX®), associated with a new extracellular autologous hepatocyte biomatrix (Porcine Autologous Biomatrix - PBM) support. To test this new bioreactor, we compared it to a standard BAL (Bio-Artificial Liver) cartridge in a ex vivo model using human plasma added to bilirubin, ammonium and lidocaine. A pathology study was performed on both bioreactors. The results suggest that ALEX® allows a maximal contact between the perfusing plasma and the liver cells and a proper hepatocyte support by a cell-to-matrix attachment. ALEX® is a suitable cell support bioreactor, guaranteeing long-term maintenance of the metabolic activity of hepatocytes when compared to a standard BAL cartridge.

Original languageEnglish (US)
Pages (from-to)960-965
Number of pages6
JournalInternational Journal of Artificial Organs
Volume25
Issue number10
StatePublished - Oct 1 2002

Keywords

  • Bioartificial Liver
  • Biomatrix
  • Bioreactor
  • Liver

ASJC Scopus subject areas

  • Biophysics

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    Ambrosino, G., Varotto, S., Basso, S., Galavotti, D., Cecchetto, A., Carraro, P., Naso, A., De Silvestro, G., Plebani, M., Giron, G., Abatangelo, G., Donato, D., Braga, G. P., Cestrone, A., Marrelli, L., Trombetta, M., Lorenzelli, V., Picardi, A., Valente, M. L., ... D'Amico, D. F. (2002). ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model. International Journal of Artificial Organs, 25(10), 960-965.