ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model

Giovanni Ambrosino; S. Varotto; S. Basso; D. Galavotti; A. Cecchetto; P. Carraro; A. Naso; G. De Silvestro; M. Plebani; G. Giron; G. Abatangelo; D. Donato; G. P. Braga; A. Cestrone; L. Marrelli; M. Trombetta; V. Lorenzelli; A. Picardi; M. L. Valente; G. Palu; A. Colantoni; D. Van Thiel; C. Ricordi; D. F. D'Amico

doi:10.1177/039139880202501010

ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model

Giovanni Ambrosino, S. Varotto, S. Basso, D. Galavotti, A. Cecchetto, P. Carraro, A. Naso, G. De Silvestro, M. Plebani, G. Giron, G. Abatangelo, D. Donato, G. P. Braga, A. Cestrone, L. Marrelli, M. Trombetta, V. Lorenzelli, A. Picardi, M. L. Valente, G. PaluA. Colantoni, D. Van Thiel, C. Ricordi, D. F. D'Amico

Surgery

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Abstract

Long-term maintenance of viability and expression of differentiated hepatocyte function is crucial for bioartificial liver support. We developed a new bioreactor design (ALEX®), associated with a new extracellular autologous hepatocyte biomatrix (Porcine Autologous Biomatrix - PBM) support. To test this new bioreactor, we compared it to a standard BAL (Bio-Artificial Liver) cartridge in a ex vivo model using human plasma added to bilirubin, ammonium and lidocaine. A pathology study was performed on both bioreactors. The results suggest that ALEX® allows a maximal contact between the perfusing plasma and the liver cells and a proper hepatocyte support by a cell-to-matrix attachment. ALEX® is a suitable cell support bioreactor, guaranteeing long-term maintenance of the metabolic activity of hepatocytes when compared to a standard BAL cartridge.

Original language	English (US)
Pages (from-to)	960-965
Number of pages	6
Journal	International Journal of Artificial Organs
Volume	25
Issue number	10
DOIs	https://doi.org/10.1177/039139880202501010
State	Published - Oct 1 2002

Keywords

Bioartificial Liver
Biomatrix
Bioreactor
Liver

ASJC Scopus subject areas

Biophysics

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10.1177/039139880202501010

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Ambrosino, G., Varotto, S., Basso, S., Galavotti, D., Cecchetto, A., Carraro, P., Naso, A., De Silvestro, G., Plebani, M., Giron, G., Abatangelo, G., Donato, D., Braga, G. P., Cestrone, A., Marrelli, L., Trombetta, M., Lorenzelli, V., Picardi, A., Valente, M. L., ... D'Amico, D. F. (2002). ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model. International Journal of Artificial Organs, 25(10), 960-965. https://doi.org/10.1177/039139880202501010

ALEX® (artificial liver for extracorporeal xenoassistance) : A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model. / Ambrosino, Giovanni; Varotto, S.; Basso, S.; Galavotti, D.; Cecchetto, A.; Carraro, P.; Naso, A.; De Silvestro, G.; Plebani, M.; Giron, G.; Abatangelo, G.; Donato, D.; Braga, G. P.; Cestrone, A.; Marrelli, L.; Trombetta, M.; Lorenzelli, V.; Picardi, A.; Valente, M. L.; Palu, G.; Colantoni, A.; Van Thiel, D.; Ricordi, C.; D'Amico, D. F.

In: International Journal of Artificial Organs, Vol. 25, No. 10, 01.10.2002, p. 960-965.

Research output: Contribution to journal › Article › peer-review

Ambrosino, G, Varotto, S, Basso, S, Galavotti, D, Cecchetto, A, Carraro, P, Naso, A, De Silvestro, G, Plebani, M, Giron, G, Abatangelo, G, Donato, D, Braga, GP, Cestrone, A, Marrelli, L, Trombetta, M, Lorenzelli, V, Picardi, A, Valente, ML, Palu, G, Colantoni, A, Van Thiel, D, Ricordi, C & D'Amico, DF 2002, 'ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model', International Journal of Artificial Organs, vol. 25, no. 10, pp. 960-965. https://doi.org/10.1177/039139880202501010

Ambrosino G, Varotto S, Basso S, Galavotti D, Cecchetto A, Carraro P et al. ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model. International Journal of Artificial Organs. 2002 Oct 1;25(10):960-965. https://doi.org/10.1177/039139880202501010

Ambrosino, Giovanni ; Varotto, S. ; Basso, S. ; Galavotti, D. ; Cecchetto, A. ; Carraro, P. ; Naso, A. ; De Silvestro, G. ; Plebani, M. ; Giron, G. ; Abatangelo, G. ; Donato, D. ; Braga, G. P. ; Cestrone, A. ; Marrelli, L. ; Trombetta, M. ; Lorenzelli, V. ; Picardi, A. ; Valente, M. L. ; Palu, G. ; Colantoni, A. ; Van Thiel, D. ; Ricordi, C. ; D'Amico, D. F. / ALEX® (artificial liver for extracorporeal xenoassistance) : A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model. In: International Journal of Artificial Organs. 2002 ; Vol. 25, No. 10. pp. 960-965.

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abstract = "Long-term maintenance of viability and expression of differentiated hepatocyte function is crucial for bioartificial liver support. We developed a new bioreactor design (ALEX{\textregistered}), associated with a new extracellular autologous hepatocyte biomatrix (Porcine Autologous Biomatrix - PBM) support. To test this new bioreactor, we compared it to a standard BAL (Bio-Artificial Liver) cartridge in a ex vivo model using human plasma added to bilirubin, ammonium and lidocaine. A pathology study was performed on both bioreactors. The results suggest that ALEX{\textregistered} allows a maximal contact between the perfusing plasma and the liver cells and a proper hepatocyte support by a cell-to-matrix attachment. ALEX{\textregistered} is a suitable cell support bioreactor, guaranteeing long-term maintenance of the metabolic activity of hepatocytes when compared to a standard BAL cartridge.",

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AU - Varotto, S.

AU - Basso, S.

AU - Galavotti, D.

AU - Cecchetto, A.

AU - Carraro, P.

AU - Naso, A.

AU - De Silvestro, G.

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AU - Giron, G.

AU - Abatangelo, G.

AU - Donato, D.

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AU - Cestrone, A.

AU - Marrelli, L.

AU - Trombetta, M.

AU - Lorenzelli, V.

AU - Picardi, A.

AU - Valente, M. L.

AU - Palu, G.

AU - Colantoni, A.

AU - Van Thiel, D.

AU - Ricordi, C.

AU - D'Amico, D. F.

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ALEX® (artificial liver for extracorporeal xenoassistance): A new bioreactor containing a porcine autologous biomatrix as hepatocyte support. Preliminary results in an ex vivo experimental model

Abstract

Keywords

ASJC Scopus subject areas

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