Alcohol-induced morphological deficits in the development of octavolateral organs of the zebrafish (Danio rerio)

Lilliann Y. Zamora, Zhongmin Lu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Prenatal alcohol exposure is known to have many profound detrimental effects on human fetal development (fetal alcohol spectrum disorders), which may manifest as lifelong disabilities. However, how alcohol affects the auditory/vestibular system is still largely unknown. This is the first study to investigate morphological effects of alcohol on the developing octavolateral system (the inner ear and lateral line) using the zebrafish, Danio rerio. Zebrafish embryos of 2 hours post fertilization (hpf) were treated in 2% alcohol for 48 hours and screened at 72 hpf for morphological defects of the inner ear and lateral line. Octavolateral organs from both alcohol-treated and control zebrafish were examined using light, confocal, and scanning electron microscopy. We observed several otolith phenotypes for alcohol-treated zebrafish including zero, one, two abnormal, two normal, and multiple otoliths. Results of this study show that alcohol treatment during early development impairs the inner ear (smaller ear, abnormal otoliths, and fewer sensory hair cells) and the lateral line (smaller neuromasts, fewer neuromasts and hair cells per neuromast, and shorter kinocilia of hair cells). Early embryonic alcohol exposure may also result in defects in hearing, balance, and hydrodynamic function of zebrafish.

Original languageEnglish
Pages (from-to)52-61
Number of pages10
JournalZebrafish
Volume10
Issue number1
DOIs
StatePublished - Mar 1 2013

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Zebrafish
Danio rerio
alcohols
Alcohols
Otolithic Membrane
ears
Inner Ear
otoliths
hairs
Fertilization
fetal alcohol syndrome
Fetal Alcohol Spectrum Disorders
fetal development
Human Development
hearing
cells
Hydrodynamics
Fetal Development
Electron Scanning Microscopy
hydrodynamics

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

Alcohol-induced morphological deficits in the development of octavolateral organs of the zebrafish (Danio rerio). / Zamora, Lilliann Y.; Lu, Zhongmin.

In: Zebrafish, Vol. 10, No. 1, 01.03.2013, p. 52-61.

Research output: Contribution to journalArticle

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