Albuminuria correlates with hemolysis and NAG and KIM-1 in patients with sickle cell anemia

Dima Hamideh, Vimal Raj, Thomas Harrington, Hua Li, Emilio Margolles, Folasade Amole, Monica Garcia-Buitrago, Phillip Ruiz, Gaston Zilleruelo, Ofelia Alvarez

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Background: Although hyperfiltration and albuminuria are common pathological conditions, kidney injury (KI) biomarkers have been seldom studied in individuals with sickle cell anemia (SCA).

Methods: We undertook a cross-sectional assessment of urine KI biomarkers in children and adults with SCA with and without albuminuria and a normal estimated glomerular filtration rate (eGFR). Albumin, KI molecule 1 (KIM-1), N-acetyl-ß-D-glucosaminidase (NAG), endothelin-1 and transforming growth factor-β1 (TGF-β1) were measured. Assays were normalized by urine creatinine. Urine intracellular hemosiderin and serum lactate dehydrogenase (LDH) were assessed as markers of hemolysis. Albuminuria was associated to the biomarkers by Pearson and Spearman correlation coefficients. Differences between the albuminuria (yes, no) groups were assessed by the t test.

Results: Nineteen patients with albuminuria (mean urine albumin/creatinine 527.14 ± 1070 mg/g, range 38.3-–190 mg/g) and 19 patients without albuminuria (mean urine albumin/creatinine 15.93 ± 5.17 mg/g, range 7.9–28.4 mg/g) were studied. The age range for the whole group was 11–48 years, and 47 % were males. Patients with albuminuria were older, had lower hematocrit, were more likely to test positive for urine hemosiderin and had a higher KIM-1 (P = 0.0035) and NAG/ creatinine ratios (P = 0.0062). Urine hemosiderin strongly correlated to a higher LDH level (P < 0.001).

Conclusions: Despite a normal or increased eGFR, KI biomarkers were detected in the urine of individuals with SCA. NAG, KIM-1 and urine hemosiderin correlated with the presence of albuminuria.

Original languageEnglish (US)
Pages (from-to)1997-2003
Number of pages7
JournalPediatric Nephrology
Issue number10
StatePublished - Oct 2014


  • Albuminuria
  • Biomarkers
  • Hemosiderin
  • Nephropathy
  • Proteinuria
  • Sickle cell anemia

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Nephrology


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