Abstract
Updated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.
| Original language | English |
|---|---|
| Pages (from-to) | 25-48 |
| Number of pages | 24 |
| Journal | Electronic Journal of Biotechnology |
| Volume | 1 |
| Issue number | 3 |
| State | Published - Dec 15 1998 |
| Externally published | Yes |
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Keywords
- Agrobacterium tumefaciens
- Plant transformation
- T-DNA
ASJC Scopus subject areas
- Biotechnology
Cite this
Agrobacterium tumefaciens : A natural tool for plant transformation. / De La Riva, Gustavo A.; González-Cabrera, Joel; Vazquez-Padron, Roberto I; Ayra-Pardo, Camilo.
In: Electronic Journal of Biotechnology, Vol. 1, No. 3, 15.12.1998, p. 25-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Agrobacterium tumefaciens
T2 - A natural tool for plant transformation
AU - De La Riva, Gustavo A.
AU - González-Cabrera, Joel
AU - Vazquez-Padron, Roberto I
AU - Ayra-Pardo, Camilo
PY - 1998/12/15
Y1 - 1998/12/15
N2 - Updated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.
AB - Updated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.
KW - Agrobacterium tumefaciens
KW - Plant transformation
KW - T-DNA
UR - http://www.scopus.com/inward/record.url?scp=0002047495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0002047495&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0002047495
VL - 1
SP - 25
EP - 48
JO - Electronic Journal of Biotechnology
JF - Electronic Journal of Biotechnology
SN - 0717-3458
IS - 3
ER -