Agrobacterium tumefaciens: A natural tool for plant transformation

Gustavo A. De La Riva, Joel González-Cabrera, Roberto Vázquez-Padrón, Camilo Ayra-Pardo

Research output: Contribution to journalReview article

89 Scopus citations

Abstract

Updated information of mechanisms for T-DNA transfer to plant cells by Agrobacterium tumefaciens is provided, focused on the role played by the different components of the virulence system. The general assessments for the establishment of efficient transformation protocols are discussed with an emphasis in the application of this methodology to monocotyledonous plants. Based on our own experience, we present the establishment of sugarcane transformation by A. tumefaciens as a model of application of this methodology to an important culture plant species, previously considered recalcitrant and inaccessible for this type of genetic manipulation.

Original languageEnglish (US)
Pages (from-to)25-48
Number of pages24
JournalElectronic Journal of Biotechnology
Volume1
Issue number3
StatePublished - Dec 15 1998
Externally publishedYes

Keywords

  • Agrobacterium tumefaciens
  • Plant transformation
  • T-DNA

ASJC Scopus subject areas

  • Biotechnology

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  • Cite this

    De La Riva, G. A., González-Cabrera, J., Vázquez-Padrón, R., & Ayra-Pardo, C. (1998). Agrobacterium tumefaciens: A natural tool for plant transformation. Electronic Journal of Biotechnology, 1(3), 25-48.