Aging in the atherosclerosis milieu may accelerate the consumption of bone marrow endothelial progenitor cells

Shoukang Zhu, Xialin Liu, Ying Li, Pascal J. Goldschmidt-Clermont, Chunming Dong

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

OBJECTIVE - We have demonstrated that bone marrow cells from young and wild-type (WT), but not old apoE, mice are capable of preventing atherosclerosis. This study was performed to elucidate the numerical and functional changes underlying the efficacy difference between young and old bone marrow. METHODS AND RESULTS - CD34/VEGFR2 conventional endothelial progenitor cells and lin/cKit/Sca-1 hematopoietic stem cells did not differ numerically or functionally between young and old apoE bone marrow. Fluorescence- activated cell sorter analysis, however, showed that a group of cells (simple little cells or SLCs), characteristically located in the lower left quadrant of forward scatter/side scatter flow cytometric plot, were markedly decreased in old WT and apoE marrow, but abundantly present in young WT and apoE bone marrow. The SLC fraction was mainly composed of lin/cKit/Sca-1 cells. In vitro differentiation assay demonstrated substantially more efficient endothelial differentiation of lin/cKit/Sca-1 SLCs than other bone marrow fractions at a single cell level and en masse. Furthermore, old lin/cKit/Sca-1 SLCs had a trend of decreased endothelial differentiation capability. CONCLUSIONS - Lin/cKit/Sca-1 SLCs may represent a previously unrecognized cell population, enriched for endothelial progenitors. The identification of these cells may help improve the efficacy of cell therapy.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume27
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Aging
  • Atherosclerosis
  • Bone marrow
  • Endothelial progenitor cells
  • Vascular repair

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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