Aggregation of chlorophylls a and b in polymer films and monolayers

Danuta Fra̧ckowiaka, Bogumił Zelent, Anouk Helluy, Małgorzata Niedbalska, Jacek Goc, Roger Leblanc

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Polarized absorption and fluorescence spectra as well as lifetime of fluorescence (τ) of the mixtures of chlorophyll a (Chl a) and chlorophyll b (Chl b) in anisotropic polyvinyl alcohol (PVA) films were measured. Two mixtures with various concentrations ratios of Chl a to Chl b were investigated. For comparison, the fluorescence excitation and emission spectra of Chl b + phytol mixture in Langmuir-Blodgett films were also measured. At high Chl a and low Chl b concentrations (MI) the following three forms of Chl a aggregates occurred: 'dry' Chl a (aggregated with polymer) with a fluorescence maximum at 676 nm (F676), hydrated dimer (Chl a 2H2O)2 or (Chl a H2O)2 (F700-705 nm), and oligomers of hydrated dimers (F750-770 nm). Chl b in the same matrix also occurs in at least three forms: 'dry' (F653 nm), 'wet' - hydrated aggregates (F662 nm) and aggregated forms with the fluorescence at 710-720 nm and/or 730-740 nm regions. The orientation of various chlorophyll forms in the PVA matrix is different. The efficiency of excitation energy transfer from Chl b to various aggregates of Chl a is different. The yield of energy transfer from Chl b to Chl a oligomers is high. Fluorescence decay of various aggregates is different and the experimental decay can be analysed always on two or three exponential components. Results are discussed on the basis of previous data concerning the monolayers of Chl a with phytol and literature data concerning aggregation of chlorophylls in various model systems. In the spectra of chlorophyll in several model systems, as follows from the literature, it is possible to distinguish the contributions from the three or even more aggregated forms of pigment similar to those reported by us. Some similarities between aggregated forms or chlorophylls and pigment forms observed in organisms are noted. In polymer films, aggregated forms of pigments are stable and oriented; therefore, their properties and the condition of their formation can be established.

Original languageEnglish
Pages (from-to)213-222
Number of pages10
JournalJournal of Photochemistry and Photobiology, A: Chemistry
Volume69
Issue number2
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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chlorophylls
Chlorophyll
Polymer films
Monolayers
Agglomeration
polymers
Fluorescence
Phytol
Pigments
Polyvinyl Alcohol
Oligomers
Dimers
Energy transfer
fluorescence
pigments
chlorophyll b
chlorophyll a
Excitation energy
Langmuir Blodgett films
polyvinyl alcohol

ASJC Scopus subject areas

  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Aggregation of chlorophylls a and b in polymer films and monolayers. / Fra̧ckowiaka, Danuta; Zelent, Bogumił; Helluy, Anouk; Niedbalska, Małgorzata; Goc, Jacek; Leblanc, Roger.

In: Journal of Photochemistry and Photobiology, A: Chemistry, Vol. 69, No. 2, 01.01.1992, p. 213-222.

Research output: Contribution to journalArticle

Fra̧ckowiaka, Danuta ; Zelent, Bogumił ; Helluy, Anouk ; Niedbalska, Małgorzata ; Goc, Jacek ; Leblanc, Roger. / Aggregation of chlorophylls a and b in polymer films and monolayers. In: Journal of Photochemistry and Photobiology, A: Chemistry. 1992 ; Vol. 69, No. 2. pp. 213-222.
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AU - Fra̧ckowiaka, Danuta

AU - Zelent, Bogumił

AU - Helluy, Anouk

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AU - Goc, Jacek

AU - Leblanc, Roger

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