Aged mice exhibit distinct B cell precursor phenotypes differing in activation, proliferation and apoptosis

Elaine Van der Put, Erin M. Sherwood, Bonnie B Blomberg, Richard L Riley

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Senescence in murine models is associated with a reduction, albeit heterogeneous, in bone marrow pre-B cells. We have categorized aged BALB/c mice into two phenotypes based on their patterns of pre-B/pro-B cell loss. Each phenotype is characterized by distinct responses to the growth cytokine IL-7 and capacity for survival in vitro. A 'moderate' loss of late-stage pre-B cells (25-80%) coincided with decline in proliferation to rmIL-7. This was also associated with a decrease in the frequency of pro-B cells which increased phosphotyrosine content upon IL-7 stimulation, an indicator of early activation events. A 'severe' loss of pre-B cells (>80%) resulted in a reduced pro-B cell pool which retained normal activation and proliferative responses to IL-7. B cell precursors from aged mice with severe alterations in B lymphopoiesis displayed increased susceptibility to apoptosis in comparison to both aged mice with moderate B cell precursor loss and young mice. Conceivably, during senescence, aged mice may initially accumulate B cell precursors which are poorly responsive to IL-7. Progressively, these refractory B cell precursors may be eliminated via apoptosis; however, the remaining limited pool of B cell precursors retains the capacity to respond to IL-7 stimulation.

Original languageEnglish
Pages (from-to)1137-1147
Number of pages11
JournalExperimental Gerontology
Volume38
Issue number10
DOIs
StatePublished - Oct 1 2003

Fingerprint

B-Lymphoid Precursor Cells
Chemical activation
Cells
Apoptosis
Phenotype
Interleukin-7
Lymphopoiesis
Phosphotyrosine
Bone Marrow Cells
Refractory materials
Bone
Cytokines

Keywords

  • Apoptosis
  • IL-7
  • Pre-B cells
  • Pro-B cells
  • Senescence

ASJC Scopus subject areas

  • Aging
  • Medicine(all)

Cite this

Aged mice exhibit distinct B cell precursor phenotypes differing in activation, proliferation and apoptosis. / Van der Put, Elaine; Sherwood, Erin M.; Blomberg, Bonnie B; Riley, Richard L.

In: Experimental Gerontology, Vol. 38, No. 10, 01.10.2003, p. 1137-1147.

Research output: Contribution to journalArticle

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