Age-dependent transcriptome and proteome following transection of neonatal spinal cord of Monodelphis domestica (South American grey short-tailed opossum)

Norman R. Saunders, Natassya M. Noor, Katarzyna M. Dziegielewska, Benjamin J. Wheaton, Shane A. Liddelow, David L. Steer, C. Joakim Ek, Mark D. Habgood, Matthew J. Wakefield, Helen Lindsay, Jessie Truettner, Robert D. Miller, A. Ian Smith, W. Dalton Dietrich

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

This study describes a combined transcriptome and proteome analysis of Monodelphis domestica response to spinal cord injury at two different postnatal ages. Previously we showed that complete transection at postnatal day 7 (P7) is followed by profuse axon growth across the lesion with near-normal locomotion and swimming when adult. In contrast, at P28 there is no axon growth across the lesion, the animals exhibit weight-bearing locomotion, but cannot use hind limbs when swimming. Here we examined changes in gene and protein expression in the segment of spinal cord rostral to the lesion at 24 h after transection at P7 and at P28. Following injury at P7 only forty genes changed (all increased expression); most were immune/inflammatory genes. Following injury at P28 many more genes changed their expression and the magnitude of change for some genes was strikingly greater. Again many were associated with the immune/inflammation response. In functional groups known to be inhibitory to regeneration in adult cords the expression changes were generally muted, in some cases opposite to that required to account for neurite inhibition. For example myelin basic protein expression was reduced following injury at P28 both at the gene and protein levels. Only four genes from families with extracellular matrix functions thought to influence neurite outgrowth in adult injured cords showed substantial changes in expression following injury at P28: Olfactomedin 4 (Olfm4, 480 fold compared to controls), matrix metallopeptidase (Mmp1, 104 fold), papilin (Papln, 152 fold) and integrin α4 (Itga4, 57 fold). These data provide a resource for investigation of a priori hypotheses in future studies of mechanisms of spinal cord regeneration in immature animals compared to lack of regeneration at more mature stages.

Original languageEnglish
Article numbere99080
JournalPLoS One
Volume9
Issue number6
DOIs
StatePublished - Jun 10 2014

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Monodelphis
Monodelphis domestica
opossums
Proteome
proteome
Spinal Cord Injuries
Transcriptome
transcriptome
spinal cord
Genes
lesions (animal)
Wounds and Injuries
Locomotion
neurites
genes
Axons
Regeneration
Animals
axons
Bearings (structural)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Age-dependent transcriptome and proteome following transection of neonatal spinal cord of Monodelphis domestica (South American grey short-tailed opossum). / Saunders, Norman R.; Noor, Natassya M.; Dziegielewska, Katarzyna M.; Wheaton, Benjamin J.; Liddelow, Shane A.; Steer, David L.; Joakim Ek, C.; Habgood, Mark D.; Wakefield, Matthew J.; Lindsay, Helen; Truettner, Jessie; Miller, Robert D.; Smith, A. Ian; Dalton Dietrich, W.

In: PLoS One, Vol. 9, No. 6, e99080, 10.06.2014.

Research output: Contribution to journalArticle

Saunders, NR, Noor, NM, Dziegielewska, KM, Wheaton, BJ, Liddelow, SA, Steer, DL, Joakim Ek, C, Habgood, MD, Wakefield, MJ, Lindsay, H, Truettner, J, Miller, RD, Smith, AI & Dalton Dietrich, W 2014, 'Age-dependent transcriptome and proteome following transection of neonatal spinal cord of Monodelphis domestica (South American grey short-tailed opossum)', PLoS One, vol. 9, no. 6, e99080. https://doi.org/10.1371/journal.pone.0099080
Saunders, Norman R. ; Noor, Natassya M. ; Dziegielewska, Katarzyna M. ; Wheaton, Benjamin J. ; Liddelow, Shane A. ; Steer, David L. ; Joakim Ek, C. ; Habgood, Mark D. ; Wakefield, Matthew J. ; Lindsay, Helen ; Truettner, Jessie ; Miller, Robert D. ; Smith, A. Ian ; Dalton Dietrich, W. / Age-dependent transcriptome and proteome following transection of neonatal spinal cord of Monodelphis domestica (South American grey short-tailed opossum). In: PLoS One. 2014 ; Vol. 9, No. 6.
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