Age-dependent disruption of basal lamina and extracellular matrix formation in l-proline analog treated otic explants

D. Berggren, Thomas R Van De Water, M. Anniko

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

l-azetidine-2-carboxylic acid, LACA, a naturally occurring vegetable imino acid, can be incorporated into mammal proteins instead of proline. This incorporation has an especially inhibitive effect on collagen secretion. Exposure of embryonic mouse inner ear explants to LACA causes dysmorphogenesis and retarded differentiation, reduces the number of collagen fibrils in the perilymphatic spaces and capsules, and gives rise to a dose-dependent derangement of the basal lamina. In control specimens, both in vivo and in vitro, the inner ear epithelia had a dense contiguous basal lamina overlying a well-developed network of collagen fibrils. When the inner ears were exposed to LACA at a concentration of 150 μg per ml of medium, there was a loss of the collagen network and gaps appeared in the basal lamina. At exposure to 300 μg LACA/ml, scarcely any collagen fibrils were present and the basal lamina was disrupted in many areas, especially beneath the sensory epithelia.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalHearing Research
Volume57
Issue number2
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Basement Membrane
Proline
Extracellular Matrix
Ear
Collagen
Inner Ear
Azetidinecarboxylic Acid
Epithelium
Imino Acids
Vegetables
Capsules
Mammals
Proteins

Keywords

  • Basal lamina
  • Collagen
  • Extracellular matrix
  • In vitro
  • l-azetidine-2-carboxylic acid
  • Otic anlage

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Age-dependent disruption of basal lamina and extracellular matrix formation in l-proline analog treated otic explants. / Berggren, D.; Van De Water, Thomas R; Anniko, M.

In: Hearing Research, Vol. 57, No. 2, 01.01.1992, p. 195-200.

Research output: Contribution to journalArticle

Berggren, D. ; Van De Water, Thomas R ; Anniko, M. / Age-dependent disruption of basal lamina and extracellular matrix formation in l-proline analog treated otic explants. In: Hearing Research. 1992 ; Vol. 57, No. 2. pp. 195-200.
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