Age-dependent changes in the proteome following complete spinal cord transection in a postnatal South American Opossum (Monodelphis domestica)

Natassya M. Noor, David L. Steer, Benjamin J. Wheaton, C. Joakim Ek, Jessie S. Truettner, W. Dalton Dietrich, Katarzyna M. Dziegielewska, Samantha J. Richardson, A. Ian Smith, John L. VandeBerg, Norman R. Saunders

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recovery from severe spinal injury in adults is limited, compared to immature animals who demonstrate some capacity for repair. Using laboratory opossums (Monodelphis domestica), the aim was to compare proteomic responses to injury at two ages: one when there is axonal growth across the lesion and substantial behavioural recovery and one when no axonal growth occurs. Anaesthetized pups at postnatal day (P) 7 or P28 were subjected to complete transection of the spinal cord at thoracic level T10. Cords were collected 1 or 7 days after injury and from age-matched controls. Proteins were separated based on isoelectric point and subunit molecular weight; those whose expression levels changed following injury were identified by densitometry and analysed by mass spectrometry. Fifty-six unique proteins were identified as differentially regulated in response to spinal transection at both ages combined. More than 50% were cytoplasmic and 70% belonged to families of proteins with characteristic binding properties. Proteins were assigned to groups by biological function including regulation (40%), metabolism (26%), inflammation (19%) and structure (15%). More changes were detected at one than seven days after injury at both ages. Seven identified proteins: 14-3-3 epsilon, 14-3-3 gamma, cofilin, alpha enolase, heart fatty acid binding protein (FABP3), brain fatty acid binding protein (FABP7) and ubiquitin demonstrated age-related differential expression and were analysed by qRT-PCR. Changes in mRNA levels for FABP3 at P7+1day and ubiquitin at P28+1day were statistically significant. Immunocytochemical staining showed differences in ubiquitin localization in younger compared to older cords and an increase in oligodendrocyte and neuroglia immunostaining following injury at P28. Western blot analysis supported proteomic results for ubiquitin and 14-3-3 proteins. Data obtained at the two ages demonstrated changes in response to injury, compared to controls, that were different for different functional protein classes. Some may provide targets for novel drug or gene therapies.

Original languageEnglish
Article numbere27465
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 16 2011

Fingerprint

Monodelphis
Monodelphis domestica
Opossums
opossums
Proteome
proteome
Spinal Cord Injuries
spinal cord
Ubiquitin
ubiquitin
Wounds and Injuries
14-3-3 Proteins
Fatty Acid-Binding Proteins
Proteins
proteins
fatty acid-binding proteins
Proteomics
Actin Depolymerizing Factors
proteomics
Drug therapy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Age-dependent changes in the proteome following complete spinal cord transection in a postnatal South American Opossum (Monodelphis domestica). / Noor, Natassya M.; Steer, David L.; Wheaton, Benjamin J.; Ek, C. Joakim; Truettner, Jessie S.; Dalton Dietrich, W.; Dziegielewska, Katarzyna M.; Richardson, Samantha J.; Smith, A. Ian; VandeBerg, John L.; Saunders, Norman R.

In: PLoS One, Vol. 6, No. 11, e27465, 16.11.2011.

Research output: Contribution to journalArticle

Noor, NM, Steer, DL, Wheaton, BJ, Ek, CJ, Truettner, JS, Dalton Dietrich, W, Dziegielewska, KM, Richardson, SJ, Smith, AI, VandeBerg, JL & Saunders, NR 2011, 'Age-dependent changes in the proteome following complete spinal cord transection in a postnatal South American Opossum (Monodelphis domestica)', PLoS One, vol. 6, no. 11, e27465. https://doi.org/10.1371/journal.pone.0027465
Noor, Natassya M. ; Steer, David L. ; Wheaton, Benjamin J. ; Ek, C. Joakim ; Truettner, Jessie S. ; Dalton Dietrich, W. ; Dziegielewska, Katarzyna M. ; Richardson, Samantha J. ; Smith, A. Ian ; VandeBerg, John L. ; Saunders, Norman R. / Age-dependent changes in the proteome following complete spinal cord transection in a postnatal South American Opossum (Monodelphis domestica). In: PLoS One. 2011 ; Vol. 6, No. 11.
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