Affinity chromatography of carbonic anhydrase

Philip L. Whitney

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

An insoluble support for affinity chromatography of carbonic anhydrase has been prepared by coupling Sulfamylon (p-aminomethylbenzene sulfonamide) to Sepharose 4B. Carbonic anhydrase binds to Sulfamylon-Sepharose very strongly and can be eluted under mild conditions by the addition of enzyme inhibitors. The gel was used to purify carbonic anhydrase from human erythrocytes and to separate isozymes B and C. It was also employed to separate native enzyme from modified carbonic anhydrases. The apoenzyme and the carboxymethyl enzyme of human carbonic anhydrase B were both isolated by this method.

Original languageEnglish (US)
Pages (from-to)467-476
Number of pages10
JournalAnalytical Biochemistry
Volume57
Issue number2
DOIs
StatePublished - Feb 1974

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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