Abstract
An insoluble support for affinity chromatography of carbonic anhydrase has been prepared by coupling Sulfamylon (p-aminomethylbenzene sulfonamide) to Sepharose 4B. Carbonic anhydrase binds to Sulfamylon-Sepharose very strongly and can be eluted under mild conditions by the addition of enzyme inhibitors. The gel was used to purify carbonic anhydrase from human erythrocytes and to separate isozymes B and C. It was also employed to separate native enzyme from modified carbonic anhydrases. The apoenzyme and the carboxymethyl enzyme of human carbonic anhydrase B were both isolated by this method.
| Original language | English |
|---|---|
| Pages (from-to) | 467-476 |
| Number of pages | 10 |
| Journal | Analytical Biochemistry |
| Volume | 57 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1974 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Affinity chromatography of carbonic anhydrase. / Whitney, Philip L.
In: Analytical Biochemistry, Vol. 57, No. 2, 01.01.1974, p. 467-476.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Affinity chromatography of carbonic anhydrase
AU - Whitney, Philip L.
PY - 1974/1/1
Y1 - 1974/1/1
N2 - An insoluble support for affinity chromatography of carbonic anhydrase has been prepared by coupling Sulfamylon (p-aminomethylbenzene sulfonamide) to Sepharose 4B. Carbonic anhydrase binds to Sulfamylon-Sepharose very strongly and can be eluted under mild conditions by the addition of enzyme inhibitors. The gel was used to purify carbonic anhydrase from human erythrocytes and to separate isozymes B and C. It was also employed to separate native enzyme from modified carbonic anhydrases. The apoenzyme and the carboxymethyl enzyme of human carbonic anhydrase B were both isolated by this method.
AB - An insoluble support for affinity chromatography of carbonic anhydrase has been prepared by coupling Sulfamylon (p-aminomethylbenzene sulfonamide) to Sepharose 4B. Carbonic anhydrase binds to Sulfamylon-Sepharose very strongly and can be eluted under mild conditions by the addition of enzyme inhibitors. The gel was used to purify carbonic anhydrase from human erythrocytes and to separate isozymes B and C. It was also employed to separate native enzyme from modified carbonic anhydrases. The apoenzyme and the carboxymethyl enzyme of human carbonic anhydrase B were both isolated by this method.
UR - http://www.scopus.com/inward/record.url?scp=0015955686&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015955686&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(74)90102-X
DO - 10.1016/0003-2697(74)90102-X
M3 - Article
C2 - 4206660
AN - SCOPUS:0015955686
VL - 57
SP - 467
EP - 476
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -