Aerobic nitroreduction of dehydrochloramphenicol by bone marrow

Mike Isildar, Wafa H. Abou-Khalil, Joaquin J Jimenez, Samir Abou-Khalil, Adel A Yunis

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

It has been previously demonstrated that dehydrochloramphenicol (DH-CAP), a bacterial metabolite of chloramphenicol, induces DNA single strand breaks in intact cells and is profoundly more cytotoxic than chloramphenicol (CAP). In view of previous observations relating genotoxicity of nitrocompounds to their nitroreduction by the target tissue, we studied the nitroreduction of DH-CAP by human and rabbit bone marrow. Nitroreduction by tissue homogenates was determined by the Bratton Marshall colorimetric assay and by high-performance liquid chromatography (HPLC). Nitroreduction of DH-CAP by bone marrow cell homogenates was observed under aerobic conditions and the reduction was both cell concentration- and time-dependent. The formation of the amino product aminodehydrochloramphenicol was confirmed by HPLC. Reduction by other tissues including human liver, Raji cells, and HL-60 tumors was also observed. These results suggest that genotoxicity of DH-CAP may be related to its nitroreduction by the target tissue with in situ production of toxic intermediates. Together with previous studies, these observations lend support to the thesis that the p-NO2 group may be the structural feature underlying aplastic anemia from CAP.

Original languageEnglish
Pages (from-to)305-310
Number of pages6
JournalToxicology and Applied Pharmacology
Volume94
Issue number2
DOIs
StatePublished - Jun 30 1988

Fingerprint

Bone
Chloramphenicol
Bone Marrow
Tissue
High performance liquid chromatography
High Pressure Liquid Chromatography
Single-Stranded DNA Breaks
Aplastic Anemia
Poisons
HL-60 Cells
Metabolites
Bone Marrow Cells
Liver
Tumors
Assays
Cells
Rabbits
dehydrochloramphenicol
DNA
Neoplasms

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Aerobic nitroreduction of dehydrochloramphenicol by bone marrow. / Isildar, Mike; Abou-Khalil, Wafa H.; Jimenez, Joaquin J; Abou-Khalil, Samir; Yunis, Adel A.

In: Toxicology and Applied Pharmacology, Vol. 94, No. 2, 30.06.1988, p. 305-310.

Research output: Contribution to journalArticle

Isildar, Mike ; Abou-Khalil, Wafa H. ; Jimenez, Joaquin J ; Abou-Khalil, Samir ; Yunis, Adel A. / Aerobic nitroreduction of dehydrochloramphenicol by bone marrow. In: Toxicology and Applied Pharmacology. 1988 ; Vol. 94, No. 2. pp. 305-310.
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