TY - JOUR
T1 - Advantage of Ischemic Preconditioning for Hepatic Resection in Pigs
AU - Kadono, Jun
AU - Hamada, Nobuo
AU - Fukueda, Mikio
AU - Ishizaki, Naoki
AU - Kaieda, Mamoru
AU - Gejima, Kentaro
AU - Nishida, Seigo
AU - Nakamura, Kazuo
AU - Yoshida, Hiroki
AU - Sakata, Ryuzo
PY - 2006/8
Y1 - 2006/8
N2 - Background: Ischemic preconditioning (IP) and intermittent inflow occlusion (IO) have provided beneficial outcomes in hepatic resection. However, comparison of these two procedures against warm hepatic ischemia-reperfusion injury has not been studied enough. Materials and methods: Pigs that had undergone 65% hepatectomy were subjected to Control (120 min continuous ischemia, n = 6), IP (10 min ischemia and 10 min reperfusion, followed by 120 min continuous ischemia, n = 6), and IO (120 min ischemia in the form of eight successive periods of 15 min ischemia and 5 min reperfusion, n = 6). We evaluated hepatocyte injury by aspartate aminotransferase, lactate dehydrogenase and hepaplastin test, hepatic microcirculation by hepatic tissue blood flow (HTBF) and endothelin (ET)-1, inflammatory response by tumor necrosis factor-α (TNF-α), and histopathology after reperfusion. Results: IP prevented hepatocyte injury, HTBF disturbance, and hepatocyte necrosis in histopathology as well as IO. These two groups showed significantly better outcomes than Control. IP produced significantly less ET-1 and TNF-α than IO. Conclusions: IP ameliorated hepatic warm ischemia-reperfusion injury. Furthermore, IP gained more advantages in preventing chemokine production such as ET-1 and inflammatory response over IO. IP could take the place of IO for hepatectomy.
AB - Background: Ischemic preconditioning (IP) and intermittent inflow occlusion (IO) have provided beneficial outcomes in hepatic resection. However, comparison of these two procedures against warm hepatic ischemia-reperfusion injury has not been studied enough. Materials and methods: Pigs that had undergone 65% hepatectomy were subjected to Control (120 min continuous ischemia, n = 6), IP (10 min ischemia and 10 min reperfusion, followed by 120 min continuous ischemia, n = 6), and IO (120 min ischemia in the form of eight successive periods of 15 min ischemia and 5 min reperfusion, n = 6). We evaluated hepatocyte injury by aspartate aminotransferase, lactate dehydrogenase and hepaplastin test, hepatic microcirculation by hepatic tissue blood flow (HTBF) and endothelin (ET)-1, inflammatory response by tumor necrosis factor-α (TNF-α), and histopathology after reperfusion. Results: IP prevented hepatocyte injury, HTBF disturbance, and hepatocyte necrosis in histopathology as well as IO. These two groups showed significantly better outcomes than Control. IP produced significantly less ET-1 and TNF-α than IO. Conclusions: IP ameliorated hepatic warm ischemia-reperfusion injury. Furthermore, IP gained more advantages in preventing chemokine production such as ET-1 and inflammatory response over IO. IP could take the place of IO for hepatectomy.
KW - hepatic resection
KW - intermittent occlusion
KW - ischemia-reperfusion injury
KW - ischemic preconditioning
UR - http://www.scopus.com/inward/record.url?scp=33746209087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746209087&partnerID=8YFLogxK
U2 - 10.1016/j.jss.2006.02.001
DO - 10.1016/j.jss.2006.02.001
M3 - Article
C2 - 16542680
AN - SCOPUS:33746209087
VL - 134
SP - 173
EP - 181
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 2
ER -