Adrenal medullary transplants reduce formalin-evoked c-fos expression in the rat spinal cord

Julie B. Siegan, Uri Herzberg, Beata R. Frydel, Jacqueline Sagen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Previous studies have indicated that adrenal medullary chromaffin cells transplanted into the spinal subarachnoid space can alleviate pain behaviors in several animal models. The goal of this study was to assess whether decreased activation of spinal dorsal horn neurons responsive to nociceptive stimuli may contribute to these antinociceptive effects. In order to address this, expression of neural activity marker c-fos in response to intraplantar formalin was evaluated in animals with intrathecal adrenal medullary or control striated muscle transplants. Adrenal medullary transplants significantly attenuated formalin-induced flinching behaviors in both acute and tonic phases of the formalin response, in comparison with control transplanted animals. Fos-like-immunoreactive (Fos-LI) cell numbers were markedly reduced in the dorsal horns of animals with adrenal medullary transplants in comparison to robust Fos-LI expression in control transplanted animals. This reduction was observed in both superficial and deep laminae of the dorsal horn, but the magnitude of the decrease was greatest in lamina V. Similar to reports using other antinociceptive treatments, some residual c-fos expression was observed, particularly in laminae I-II, in animals with adrenal medullary transplants. The results of these studies suggest that adrenal medullary transplants produce antinociception in part by inhibiting spinal dorsal horn neuronal activation in response to noxious stimuli.

Original languageEnglish
Pages (from-to)174-183
Number of pages10
JournalBrain Research
Volume944
Issue number1-2
DOIs
StatePublished - Jul 19 2002

Fingerprint

Formaldehyde
Spinal Cord
Transplants
Posterior Horn Cells
Chromaffin Cells
Subarachnoid Space
Striated Muscle
Animal Models
Cell Count
Pain
Spinal Cord Dorsal Horn
Therapeutics

Keywords

  • Antinociception
  • Chromaffin cell
  • Dorsal horn
  • Fos
  • Neural transplant

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Adrenal medullary transplants reduce formalin-evoked c-fos expression in the rat spinal cord. / Siegan, Julie B.; Herzberg, Uri; Frydel, Beata R.; Sagen, Jacqueline.

In: Brain Research, Vol. 944, No. 1-2, 19.07.2002, p. 174-183.

Research output: Contribution to journalArticle

Siegan, Julie B. ; Herzberg, Uri ; Frydel, Beata R. ; Sagen, Jacqueline. / Adrenal medullary transplants reduce formalin-evoked c-fos expression in the rat spinal cord. In: Brain Research. 2002 ; Vol. 944, No. 1-2. pp. 174-183.
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