Adrenal medullary transplants increase spinal cord cerebrospinal fluid catecholamine levels and reduce pain sensitivity

Jacqueline Sagen, James E. Kemmler, Hong Wang

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Previous work in this laboratory has shown that adrenal medullary transplants into the spinal cord subarachnoid space can reduce pain sensitivity. This analgesia most likely results from the release of neuroactive substances, particularly catecholamines and opioid peptides, from the transplanted cells into the CSF of the spinal cord, since it can be attenuated or blocked by α-adrenergic or opiate antagonists. The purpose of the present study was to more directly measure the release of catecholamines from adrenal medullary transplants in the spinal cord CSF using a spinal superfusion technique. CSF samples from rats with 6-month-old transplants were assayed for catecholamines using HPLC with electrochemical detection. Results indicated that norepinephrine levels were increased threefold, and epinephrine levels nearly 100-fold, in animals with adrenal medullary transplants compared with control transplanted animals. There was no apparent increase in dopamine levels. Furthermore, the increased levels of total catecholamines were correlated with decreased pain sensitivity. Results of this study indicate that adrenal medullary transplants can survive for long periods in the rat spinal CSF and continue to release high levels of catecholamines. Together, the release of catecholamines and opioid peptides from adrenal medullary transplants may provide the ideal combination for the reduction of pain.

Original languageEnglish
Pages (from-to)623-627
Number of pages5
JournalJournal of Neurochemistry
Volume56
Issue number2
StatePublished - Dec 1 1991
Externally publishedYes

Fingerprint

Cerebrospinal fluid
Transplants
Catecholamines
Cerebrospinal Fluid
Spinal Cord
Pain
Opioid Peptides
Rats
Opiate Alkaloids
Animals
Subarachnoid Space
Adrenergic Agents
Analgesia
Epinephrine
Dopamine
Norepinephrine
High Pressure Liquid Chromatography

Keywords

  • Analgesia
  • Chromaffin cells
  • Epinephrine
  • Neural grafts
  • Nociception
  • Norepinephrine

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Adrenal medullary transplants increase spinal cord cerebrospinal fluid catecholamine levels and reduce pain sensitivity. / Sagen, Jacqueline; Kemmler, James E.; Wang, Hong.

In: Journal of Neurochemistry, Vol. 56, No. 2, 01.12.1991, p. 623-627.

Research output: Contribution to journalArticle

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