Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females

Sebastian D. Parlee, Becky R. Simon, Erica L. Scheller, Emilyn U. Alejandro, Brian S. Learman, Venkatesh Krishnan, Ernesto Bernal Mizrachi, Ormond A. MacDougald

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Nutritional or pharmacological perturbations during perinatal growth can cause persistent effects on the function of white adipose tissue, altering susceptibility to obesity later in life. Previous studies have established that saccharin, a nonnutritive sweetener, inhibits lipolysis in mature adipocytes and stimulates adipogenesis. Thus, the current study tested whether neonatal exposure to saccharin via maternal lactation increased susceptibility of mice to diet-induced obesity. Saccharin decreased body weight of female mice beginning postnatal week 3. Decreased liver weights on week 14 corroborated this diminished body weight. Initially, saccharin also reduced male mouse body weight. By week 5, weights transiently rebounded above controls, and by week 14, male body weights did not differ. Body composition analysis revealed that saccharin increased lean and decreased fat mass of male mice, the latter due to decreased adipocyte size and epididymal, perirenal, and sc adipose weights. A mild improvement in glucose tolerance without a change in insulin sensitivity or secretion aligned with this leaner phenotype. Interestingly, microcomputed tomography analysis indicated that saccharin also increased cortical and trabecular bone mass of male mice and modified cortical bone alone in female mice. A modest increase in circulating testosterone may contribute to the leaner phenotype in male mice. Accordingly, the current study established a developmental period in which saccharin at high concentrations reduces adiposity and increases lean and bone mass in male mice while decreasing generalized growth in female mice.

Original languageEnglish (US)
Pages (from-to)1313-1326
Number of pages14
JournalEndocrinology
Volume155
Issue number4
DOIs
StatePublished - Apr 2014
Externally publishedYes

Fingerprint

Saccharin
Body Composition
Body Weight
Adipocytes
Weights and Measures
Obesity
Phenotype
Sweetening Agents
X-Ray Microtomography
Adipogenesis
White Adipose Tissue
Lipolysis
Adiposity
Growth
Lactation
Insulin Resistance
Testosterone
Fats
Mothers
Pharmacology

ASJC Scopus subject areas

  • Endocrinology

Cite this

Parlee, S. D., Simon, B. R., Scheller, E. L., Alejandro, E. U., Learman, B. S., Krishnan, V., ... MacDougald, O. A. (2014). Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females. Endocrinology, 155(4), 1313-1326. https://doi.org/10.1210/en.2013-1995

Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females. / Parlee, Sebastian D.; Simon, Becky R.; Scheller, Erica L.; Alejandro, Emilyn U.; Learman, Brian S.; Krishnan, Venkatesh; Bernal Mizrachi, Ernesto; MacDougald, Ormond A.

In: Endocrinology, Vol. 155, No. 4, 04.2014, p. 1313-1326.

Research output: Contribution to journalArticle

Parlee, SD, Simon, BR, Scheller, EL, Alejandro, EU, Learman, BS, Krishnan, V, Bernal Mizrachi, E & MacDougald, OA 2014, 'Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females', Endocrinology, vol. 155, no. 4, pp. 1313-1326. https://doi.org/10.1210/en.2013-1995
Parlee, Sebastian D. ; Simon, Becky R. ; Scheller, Erica L. ; Alejandro, Emilyn U. ; Learman, Brian S. ; Krishnan, Venkatesh ; Bernal Mizrachi, Ernesto ; MacDougald, Ormond A. / Administration of saccharin to neonatal mice influences body composition of adult males and reduces body weight of females. In: Endocrinology. 2014 ; Vol. 155, No. 4. pp. 1313-1326.
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