Administration and 1H MRS, detection of histidine in human brain: Application to in vivo pH measurement

Peter Vermathen, Aristides A. Capizzano, Andrew A. Maudsley

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Measurement of histidine in vivo offers the potential for tissue pH measurement using routinely performed 1H MR spectroscopy. In the brain, however, histidine concentrations are generally too low for reliable measurement. By using oral loading of histidine, this study demonstrates that brain concentrations can be significantly increased, enabling detection of histidine by localized 1H MR measurements and making in vivo pH measurement possible. In studies carried out on healthy human subjects at 1.5 T, a consistent spectral quality downfield from water was achieved using a PRESS sequence at short echo times. Measurements at different TE values helped to characterize the downfield spectral region. Histidine loading of 400 mg/kg of body weight increased brain histidine levels by approximately 0.8 mM, with maximum histidine concentration reached 4 to 7 hr after consumption. The pH calculated from histidine resonances was 6.96, and a hyperventilation study demonstrated the potential for measuring altered pH. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)665-675
Number of pages11
JournalMagnetic Resonance in Medicine
Issue number5
StatePublished - 2000
Externally publishedYes


  • Histidine
  • Human brain
  • In vivo proton MR spectroscopy
  • pH

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology


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