Adipose-derived stromal cell therapy improves cardiac function after coronary Occlusion in rats

Luiza L.S. Bagno, João Pedro S. Werneck-de-Castro, Patrícia F. Oliveira, Márcia S. Cunha-Abreu, Nazareth N. Rocha, Taís H. Kasai-Brunswick, Vivian M. Lago, Regina C.S. Goldenberg, Antonio C. Campos-de-Carvalho

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Recent studies have identified adipose tissue as a new source of mesenchymal stem cells for therapy. The purpose of this study was to investigate the therapy with adipose-derived stromal cells (ASCs) in a rat model of healed myocardial infarction (MI). ASCs from inguinal subcutaneous adipose tissue of male Wistar rats were isolated by enzymatic digestion and filtration. Cells were then cultured until passage 3. Four weeks after ligation of the left coronary artery of female rats, a suspension of either 100 μl with phosphate-buffered saline (PBS) + Matrigel + 2 × 106 ASCs labeled with Hoechst (n = 11) or 100 μl of PBS + Matrigel (n = 10) was injected along the borders of the ventricular wall scar tissue. A sham-operated group (n = 5) was submitted to the same surgical procedure except permanent ligation of left coronary artery. Cardiac performance was assessed by electro- and echocardiogram. Echo was performed prior to injections (baseline, BL) and 6 weeks after injections (follow-up, FU), and values after treatment were normalized by values obtained before treatment. Hemodynamic measurements were performed 6 weeks after injections. All infarcted animals exhibited cardiac function impairment. Ejection fraction (EF), shortening fractional area (SFA), and left ventricular akinesia (LVA) were similar between infarcted groups before treatment. Six weeks after therapy, ASC group showed significant improvement in all three ECHO indices in comparison to vehicle group. In anesthetized animals dp/dt+ was also significantly higher in ASCs when compared to vehicle. In agreement with functional improvement, scar area was diminished in the ASC group. We conclude that ASCs improve cardiac function in infarcted rats when administered directly to the myocardium.

Original languageEnglish (US)
Pages (from-to)1985-1996
Number of pages12
JournalCell transplantation
Volume21
Issue number9
DOIs
StatePublished - 2012
Externally publishedYes

Keywords

  • Adipose-derived stromal cell
  • Cell therapy and heart healing
  • Myocardial infarction

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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