Adhesion forces between individual ligand-receptor pairs

Ernst Ludwig Florin, Vincent T. Moy, Hermann E. Gaub

Research output: Contribution to journalArticle

1622 Citations (Scopus)

Abstract

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 ± 20 piconewtons for biotin and 85 ± 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.

Original languageEnglish
Pages (from-to)415-417
Number of pages3
JournalScience
Volume264
Issue number5157
StatePublished - Apr 15 1994
Externally publishedYes

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Biotin
Ligands
iminobiotin
avidin-agarose
desthiobiotin

ASJC Scopus subject areas

  • General

Cite this

Florin, E. L., Moy, V. T., & Gaub, H. E. (1994). Adhesion forces between individual ligand-receptor pairs. Science, 264(5157), 415-417.

Adhesion forces between individual ligand-receptor pairs. / Florin, Ernst Ludwig; Moy, Vincent T.; Gaub, Hermann E.

In: Science, Vol. 264, No. 5157, 15.04.1994, p. 415-417.

Research output: Contribution to journalArticle

Florin, EL, Moy, VT & Gaub, HE 1994, 'Adhesion forces between individual ligand-receptor pairs', Science, vol. 264, no. 5157, pp. 415-417.
Florin EL, Moy VT, Gaub HE. Adhesion forces between individual ligand-receptor pairs. Science. 1994 Apr 15;264(5157):415-417.
Florin, Ernst Ludwig ; Moy, Vincent T. ; Gaub, Hermann E. / Adhesion forces between individual ligand-receptor pairs. In: Science. 1994 ; Vol. 264, No. 5157. pp. 415-417.
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