Adhesion forces between individual ligand-receptor pairs

Ernst Ludwig Florin, Vincent T. Moy, Hermann E. Gaub

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Abstract

The adhesion force between the tip of an atomic force microscope cantilever derivatized with avidin and agarose beads functionalized with biotin, desthiobiotin, or iminobiotin was measured. Under conditions that allowed only a limited number of molecular pairs to interact, the force required to separate tip and bead was found to be quantized in integer multiples of 160 ± 20 piconewtons for biotin and 85 ± 15 piconewtons for iminobiotin. The measured force quanta are interpreted as the unbinding forces of individual molecular pairs.

Original languageEnglish
Pages (from-to)415-417
Number of pages3
JournalScience
Volume264
Issue number5157
StatePublished - Apr 15 1994
Externally publishedYes

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Cite this

Florin, E. L., Moy, V. T., & Gaub, H. E. (1994). Adhesion forces between individual ligand-receptor pairs. Science, 264(5157), 415-417.