Adenovirus infection in pediatric liver and intestinal transplant recipients

Utility of DNA detection by PCR

Gwenn E McLaughlin, Spiros Delis, Lutifat Kashimawo, G. Patricia Cantwell, Naveen Mittal, Robert E. Cirocco, Phillip Ruiz, Tomoaki Kato, Andreas G. Tzakis

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

To evaluate the incidence of adenovirus (AdV) infection in pediatric liver and intestinal transplant recipients, the records of patients with possible AdV infection were reviewed for demographic data, symptomatology, methods of diagnosis, treatment and outcome. To evaluate the impact of polymerase chain reaction (PCR) amplification and identification of AdV DNA as a diagnostic test, the incidence and outcome of AdV before and after the introduction of PCR were compared. Adenovirus infection was identified in 4.1% of liver recipients and 20.8% of intestinal transplant recipients. The overall incidence of AdV did not increase over time, even following the introduction of PCR for virus detection. The higher incidence of AdV in the pediatric intestinal transplant recipients may be attributed to the frequent application of PCR methodology to intestinal biopsy material. Detection of AdV by PCR was associated with reduced mortality compared with detection by culture, either because of earlier detection of invasive disease or because PCR detects the presence of latent as well as active AdV.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalAmerican Journal of Transplantation
Volume3
Issue number2
DOIs
StatePublished - Feb 1 2003

Fingerprint

Adenoviridae Infections
DNA-Directed DNA Polymerase
Adenoviridae
Pediatrics
Polymerase Chain Reaction
Liver
Incidence
Routine Diagnostic Tests
Transplant Recipients
Early Diagnosis
Demography
Viruses
Biopsy
Mortality
DNA

Keywords

  • Abdominal transplants
  • Adenovirus
  • Pediatric
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Immunology

Cite this

Adenovirus infection in pediatric liver and intestinal transplant recipients : Utility of DNA detection by PCR. / McLaughlin, Gwenn E; Delis, Spiros; Kashimawo, Lutifat; Patricia Cantwell, G.; Mittal, Naveen; Cirocco, Robert E.; Ruiz, Phillip; Kato, Tomoaki; Tzakis, Andreas G.

In: American Journal of Transplantation, Vol. 3, No. 2, 01.02.2003, p. 224-228.

Research output: Contribution to journalArticle

McLaughlin, Gwenn E ; Delis, Spiros ; Kashimawo, Lutifat ; Patricia Cantwell, G. ; Mittal, Naveen ; Cirocco, Robert E. ; Ruiz, Phillip ; Kato, Tomoaki ; Tzakis, Andreas G. / Adenovirus infection in pediatric liver and intestinal transplant recipients : Utility of DNA detection by PCR. In: American Journal of Transplantation. 2003 ; Vol. 3, No. 2. pp. 224-228.
@article{b9c2e0df1a7b4c249bd0053463983c1d,
title = "Adenovirus infection in pediatric liver and intestinal transplant recipients: Utility of DNA detection by PCR",
abstract = "To evaluate the incidence of adenovirus (AdV) infection in pediatric liver and intestinal transplant recipients, the records of patients with possible AdV infection were reviewed for demographic data, symptomatology, methods of diagnosis, treatment and outcome. To evaluate the impact of polymerase chain reaction (PCR) amplification and identification of AdV DNA as a diagnostic test, the incidence and outcome of AdV before and after the introduction of PCR were compared. Adenovirus infection was identified in 4.1{\%} of liver recipients and 20.8{\%} of intestinal transplant recipients. The overall incidence of AdV did not increase over time, even following the introduction of PCR for virus detection. The higher incidence of AdV in the pediatric intestinal transplant recipients may be attributed to the frequent application of PCR methodology to intestinal biopsy material. Detection of AdV by PCR was associated with reduced mortality compared with detection by culture, either because of earlier detection of invasive disease or because PCR detects the presence of latent as well as active AdV.",
keywords = "Abdominal transplants, Adenovirus, Pediatric, Polymerase chain reaction",
author = "McLaughlin, {Gwenn E} and Spiros Delis and Lutifat Kashimawo and {Patricia Cantwell}, G. and Naveen Mittal and Cirocco, {Robert E.} and Phillip Ruiz and Tomoaki Kato and Tzakis, {Andreas G.}",
year = "2003",
month = "2",
day = "1",
doi = "10.1034/j.1600-6143.2003.00007.x",
language = "English",
volume = "3",
pages = "224--228",
journal = "American Journal of Transplantation",
issn = "1600-6135",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Adenovirus infection in pediatric liver and intestinal transplant recipients

T2 - Utility of DNA detection by PCR

AU - McLaughlin, Gwenn E

AU - Delis, Spiros

AU - Kashimawo, Lutifat

AU - Patricia Cantwell, G.

AU - Mittal, Naveen

AU - Cirocco, Robert E.

AU - Ruiz, Phillip

AU - Kato, Tomoaki

AU - Tzakis, Andreas G.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - To evaluate the incidence of adenovirus (AdV) infection in pediatric liver and intestinal transplant recipients, the records of patients with possible AdV infection were reviewed for demographic data, symptomatology, methods of diagnosis, treatment and outcome. To evaluate the impact of polymerase chain reaction (PCR) amplification and identification of AdV DNA as a diagnostic test, the incidence and outcome of AdV before and after the introduction of PCR were compared. Adenovirus infection was identified in 4.1% of liver recipients and 20.8% of intestinal transplant recipients. The overall incidence of AdV did not increase over time, even following the introduction of PCR for virus detection. The higher incidence of AdV in the pediatric intestinal transplant recipients may be attributed to the frequent application of PCR methodology to intestinal biopsy material. Detection of AdV by PCR was associated with reduced mortality compared with detection by culture, either because of earlier detection of invasive disease or because PCR detects the presence of latent as well as active AdV.

AB - To evaluate the incidence of adenovirus (AdV) infection in pediatric liver and intestinal transplant recipients, the records of patients with possible AdV infection were reviewed for demographic data, symptomatology, methods of diagnosis, treatment and outcome. To evaluate the impact of polymerase chain reaction (PCR) amplification and identification of AdV DNA as a diagnostic test, the incidence and outcome of AdV before and after the introduction of PCR were compared. Adenovirus infection was identified in 4.1% of liver recipients and 20.8% of intestinal transplant recipients. The overall incidence of AdV did not increase over time, even following the introduction of PCR for virus detection. The higher incidence of AdV in the pediatric intestinal transplant recipients may be attributed to the frequent application of PCR methodology to intestinal biopsy material. Detection of AdV by PCR was associated with reduced mortality compared with detection by culture, either because of earlier detection of invasive disease or because PCR detects the presence of latent as well as active AdV.

KW - Abdominal transplants

KW - Adenovirus

KW - Pediatric

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0037298410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037298410&partnerID=8YFLogxK

U2 - 10.1034/j.1600-6143.2003.00007.x

DO - 10.1034/j.1600-6143.2003.00007.x

M3 - Article

VL - 3

SP - 224

EP - 228

JO - American Journal of Transplantation

JF - American Journal of Transplantation

SN - 1600-6135

IS - 2

ER -