Adenoviral capsid modulates secretory compartment organization and function in acinar epithelial cells from rabbit lacrimal gland

Y. Wang, J. Xie, F. A. Yarber, C. Mazurek, M. D. Trousdale, L. K. Kauwe-Medina, Noriyuki Kasahara, S. F. Hamm-Alvarez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although adenovirus (Ad) exhibits tropism for epithelial cells, little is known about the cellular effects of adenoviral binding and internalization on epithelial functions. Here, we examine its effects on the secretory acinar epithelial cells of the lacrimal gland, responsible for stimulated release of tear proteins into ocular fluid. Exposure of reconstituted rabbit lacrimal acini to replication-defective Ad for 16-18 h under conditions that resulted in >80% transduction efficiency did not alter cytoskeletal filament or biosynthetic/endosomal membrane compartment organization. Transduction specifically altered the organization of the stimulated secretory pathway, eliciting major dispersal of rab3D immunofluorescence from apical stores normally associated with mature secretory vesicles. Biochemical studies revealed that this dispersal was not associated with altered rab3D expression nor its release from cellular membranes. Ultraviolet (UV)-inactivated Ad elicited similar dispersal of rab3D immunofluorescence. In acini exposed to replication-defective or UV-inactivated Ad, carbachol-stimulated release of bulk protein and β-hexosaminidase were significantly (P≤0.05) inhibited to an extent proportional to the loss of rab3D-enriched mature secretory vesicles associated with these treatments. We propose that the altered secretory compartment organization and function caused by Ad reflects changes in the normal maturation of secretory vesicles, and that these changes are caused by exposure to the Ad capsid.

Original languageEnglish (US)
Pages (from-to)970-981
Number of pages12
JournalGene Therapy
Volume11
Issue number12
DOIs
StatePublished - Jun 2004
Externally publishedYes

Fingerprint

Lacrimal Apparatus
Acinar Cells
Capsid
Adenoviridae
Epithelial Cells
Rabbits
Secretory Vesicles
Fluorescent Antibody Technique
Hexosaminidases
Tropism
Membranes
Secretory Pathway
Carbachol
Cytoskeleton
Tears
Proteins

Keywords

  • Adenovirus
  • Adenovirus capsid
  • Exocytosis
  • Lacrimal gland
  • Rab3D

ASJC Scopus subject areas

  • Genetics

Cite this

Wang, Y., Xie, J., Yarber, F. A., Mazurek, C., Trousdale, M. D., Kauwe-Medina, L. K., ... Hamm-Alvarez, S. F. (2004). Adenoviral capsid modulates secretory compartment organization and function in acinar epithelial cells from rabbit lacrimal gland. Gene Therapy, 11(12), 970-981. https://doi.org/10.1038/sj.gt.3302247

Adenoviral capsid modulates secretory compartment organization and function in acinar epithelial cells from rabbit lacrimal gland. / Wang, Y.; Xie, J.; Yarber, F. A.; Mazurek, C.; Trousdale, M. D.; Kauwe-Medina, L. K.; Kasahara, Noriyuki; Hamm-Alvarez, S. F.

In: Gene Therapy, Vol. 11, No. 12, 06.2004, p. 970-981.

Research output: Contribution to journalArticle

Wang, Y, Xie, J, Yarber, FA, Mazurek, C, Trousdale, MD, Kauwe-Medina, LK, Kasahara, N & Hamm-Alvarez, SF 2004, 'Adenoviral capsid modulates secretory compartment organization and function in acinar epithelial cells from rabbit lacrimal gland', Gene Therapy, vol. 11, no. 12, pp. 970-981. https://doi.org/10.1038/sj.gt.3302247
Wang, Y. ; Xie, J. ; Yarber, F. A. ; Mazurek, C. ; Trousdale, M. D. ; Kauwe-Medina, L. K. ; Kasahara, Noriyuki ; Hamm-Alvarez, S. F. / Adenoviral capsid modulates secretory compartment organization and function in acinar epithelial cells from rabbit lacrimal gland. In: Gene Therapy. 2004 ; Vol. 11, No. 12. pp. 970-981.
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