Adenosine deaminase enhances T-cell response elicited by dendritic cells loaded with inactivated HIV

Núria Climent, José M. Martinez-Navio, Cristina Gil, Felipe Garcia, Cristina Rovira, Carmen Hurtado, Laia Miralles, José M. Gatell, Teresa Gallart, Josefa Mallol, Carme Lluis, Rafael Franco

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

As host immunological defenses are impaired during HIV infection, it is difficult to elicit good responses when attempting to develop therapeutic vaccines against HIV. To try to solve this situation, adjuvants, particularly cytokines, are currently under evaluation. Owing to the fact that adenosine deaminase (ADA) is a member of the family of growth factor with deaminase activity, we tested whether it could improve immune responses in the development of HIV dendritic-cell-based therapeutic vaccines. A co-culture model approach has been used to test the usefulness of ADA as adjuvant. Monocyte-derived dendritic cells from HIV-infected patients were pulsed with inactivated HIV, matured and co-cultured with autologous T cells. Addition of ADA to the co-cultures resulted in enhanced CD4+ and CD8+ T-cell proliferation and robust ADA-induced increase in cytokine production (IFN-γ, TNF-α and IL-6). As IFN-γ, TNF-α and IL-6 promote the Th1 versus Th2 phenotype and improve T helper proliferation responses and antigen-specific CTL responses ADA may be considered a promising candidate for therapeutic vaccine adjuvant.

Original languageEnglish (US)
Pages (from-to)634-639
Number of pages6
JournalImmunology and Cell Biology
Volume87
Issue number8
DOIs
StatePublished - Nov 2009
Externally publishedYes

Keywords

  • Adenosine deaminase
  • Anti-HIV response
  • Cytokines as vaccine adjuvant
  • Dendritic cells
  • T-cell proliferation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Cell Biology

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