Adenosine, a "retaliatory" metabolite, promotes anoxia tolerance in turtle brain

Miguel Perez-Pinzon, Peter L. Lutz, Thomas Sick, Myron Rosenthal

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Contrary to what is found in most vertebrates, the brains of certain turtle species maintain ATP levels and ion homeostasis and survive prolonged anoxia. The hypothesis tested here is that the release of adenosine and its binding to A1 receptors are essential for this anoxic tolerance. Studies were conducted in the isolated turtle cerebellum, which did release adenosine to the extracellular space during anoxia. When adenosine receptor antagonists [theophylline, 8-cyclopentyltheophylline (CPT), or 8-cyclopentyl-l,3-dipropylxanthine (DPCPX)] were added to the superfusate under control conditions, they had no effect on extracellular potassium ion activity ([K + ]0. During anoxia, however, these antagonists provoked maximal efflux of K + (anoxic depolarization). Anoxic depolarization occurred earlier during anoxia with theophylline (a nonspecific adenosine receptor antagonist) than with CPT or DPCPX, which specifically block A1 receptors. Therefore, adenosine release and effects mediated by A1 receptors are essential to anoxia tolerance in turtle brain.

Original languageEnglish
Pages (from-to)728-732
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume13
Issue number4
StatePublished - Dec 1 1993

Fingerprint

Turtles
Adenosine
Purinergic P1 Receptor Antagonists
Brain
Theophylline
Ions
Extracellular Space
Cerebellum
Vertebrates
Potassium
Homeostasis
Adenosine Triphosphate
Hypoxia

Keywords

  • A receptors
  • Adenosine
  • Anoxia
  • Cerebellum
  • Sodium channels
  • Turtle

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

Cite this

Adenosine, a "retaliatory" metabolite, promotes anoxia tolerance in turtle brain. / Perez-Pinzon, Miguel; Lutz, Peter L.; Sick, Thomas; Rosenthal, Myron.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 13, No. 4, 01.12.1993, p. 728-732.

Research output: Contribution to journalArticle

Perez-Pinzon, Miguel ; Lutz, Peter L. ; Sick, Thomas ; Rosenthal, Myron. / Adenosine, a "retaliatory" metabolite, promotes anoxia tolerance in turtle brain. In: Journal of Cerebral Blood Flow and Metabolism. 1993 ; Vol. 13, No. 4. pp. 728-732.
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