Acute toxicity, accumulation and tissue distribution of copper in the blue crab Callinectes sapidus acclimated to different salinities

In vivo and in vitro studies

Camila De Martinez Gaspar Martins, Indianara Fernanda Barcarolli, Eliana Jaime de Menezes, Marina Mussoi Giacomin, Chris M. Wood, Adalto Bianchini

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

In vivo and in vitro studies were performed to evaluate acute toxicity, organ-specific distribution, and tissue accumulation of copper in Callinectes sapidus acclimated to two different experimental salinities (2 and 30ppt). Blue crabs were quite tolerant to copper. Acute dissolved copper toxicity (96-h LC50 and its corresponding 95% confident interval) was higher at salinity 2ppt (5.3 (3.50-8.05)μM Cu) than at 30ppt (53.0 (27.39-102.52)μM Cu). The difference between salinities can be completely explained based on the water chemistry because it disappeared when 96-h LC50 values were expressed as the free Cu2+ ion (3.1 (1.93-4.95)μM free Cu at 2ppt versus 5.6 (2.33-13.37)μM free Cu at 30ppt) or the Cu2+ activity (1.4 (0.88-2.26)μM Cu activity at 2ppt versus 1.7 (0.71-4.07)μM Cu activity at 30ppt). The relationships between gill Cu burden and % mortality were very similar at 2 and 30ppt, in accord with the Biotic Ligand Model. In vivo experiments showed that copper concentration in the hemolymph is not dependent on metal concentration in the surrounding medium at either experimental salinity. They also showed that copper flux into the gills is higher than into other tissues analyzed, and that anterior and posterior gills are similarly important sites of copper accumulation at both experimental salinities. In vitro experiments with isolated-perfused gills showed that there is a positive relationship between copper accumulation in this tissue and the metal concentration in the incubation media for both anterior and posterior gills. A similar result was observed at both low and high salinities. Furthermore, in vitro experiments showed that copper accumulation in posterior gills is also positively and strongly dependent on the incubation time with copper. Gill copper accumulation occurred at a lower rate in the first 2h of metal exposure, increasing markedly after this " steady-state" period. This finding was corroborated by a significant increase in copper influx to the gill perfusate (corresponding to crab hemolymph) after this time, measured using 64Cu. In vivo, after uptake from solution, 64Cu was primarily accumulated in the gills and the rest of the body rather than in the hemolymph, hepatopancreas, or other internal tissues. Overall, the present findings indicate that gills are a key target organ for copper accumulation, as well as an important biological barrier against the excessive uptake of copper into the hemolymph and the subsequent distribution of this metal to internal organs of the blue crab.

Original languageEnglish
Pages (from-to)88-99
Number of pages12
JournalAquatic Toxicology
Volume101
Issue number1
DOIs
StatePublished - Jan 17 2011

Fingerprint

Brachyura
Callinectes sapidus
Salinity
tissue distribution
Tissue Distribution
acute toxicity
in vitro studies
Copper
crab
crabs
copper
salinity
toxicity
gills
Hemolymph
hemolymph
Metals
metals
metal
lethal concentration 50

Keywords

  • Accumulation
  • Acute toxicity
  • Blue crab
  • Copper
  • Gills
  • Organ-specific distribution
  • Salinity

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Acute toxicity, accumulation and tissue distribution of copper in the blue crab Callinectes sapidus acclimated to different salinities : In vivo and in vitro studies. / Martins, Camila De Martinez Gaspar; Barcarolli, Indianara Fernanda; de Menezes, Eliana Jaime; Giacomin, Marina Mussoi; Wood, Chris M.; Bianchini, Adalto.

In: Aquatic Toxicology, Vol. 101, No. 1, 17.01.2011, p. 88-99.

Research output: Contribution to journalArticle

Martins, Camila De Martinez Gaspar ; Barcarolli, Indianara Fernanda ; de Menezes, Eliana Jaime ; Giacomin, Marina Mussoi ; Wood, Chris M. ; Bianchini, Adalto. / Acute toxicity, accumulation and tissue distribution of copper in the blue crab Callinectes sapidus acclimated to different salinities : In vivo and in vitro studies. In: Aquatic Toxicology. 2011 ; Vol. 101, No. 1. pp. 88-99.
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N2 - In vivo and in vitro studies were performed to evaluate acute toxicity, organ-specific distribution, and tissue accumulation of copper in Callinectes sapidus acclimated to two different experimental salinities (2 and 30ppt). Blue crabs were quite tolerant to copper. Acute dissolved copper toxicity (96-h LC50 and its corresponding 95% confident interval) was higher at salinity 2ppt (5.3 (3.50-8.05)μM Cu) than at 30ppt (53.0 (27.39-102.52)μM Cu). The difference between salinities can be completely explained based on the water chemistry because it disappeared when 96-h LC50 values were expressed as the free Cu2+ ion (3.1 (1.93-4.95)μM free Cu at 2ppt versus 5.6 (2.33-13.37)μM free Cu at 30ppt) or the Cu2+ activity (1.4 (0.88-2.26)μM Cu activity at 2ppt versus 1.7 (0.71-4.07)μM Cu activity at 30ppt). The relationships between gill Cu burden and % mortality were very similar at 2 and 30ppt, in accord with the Biotic Ligand Model. In vivo experiments showed that copper concentration in the hemolymph is not dependent on metal concentration in the surrounding medium at either experimental salinity. They also showed that copper flux into the gills is higher than into other tissues analyzed, and that anterior and posterior gills are similarly important sites of copper accumulation at both experimental salinities. In vitro experiments with isolated-perfused gills showed that there is a positive relationship between copper accumulation in this tissue and the metal concentration in the incubation media for both anterior and posterior gills. A similar result was observed at both low and high salinities. Furthermore, in vitro experiments showed that copper accumulation in posterior gills is also positively and strongly dependent on the incubation time with copper. Gill copper accumulation occurred at a lower rate in the first 2h of metal exposure, increasing markedly after this " steady-state" period. This finding was corroborated by a significant increase in copper influx to the gill perfusate (corresponding to crab hemolymph) after this time, measured using 64Cu. In vivo, after uptake from solution, 64Cu was primarily accumulated in the gills and the rest of the body rather than in the hemolymph, hepatopancreas, or other internal tissues. Overall, the present findings indicate that gills are a key target organ for copper accumulation, as well as an important biological barrier against the excessive uptake of copper into the hemolymph and the subsequent distribution of this metal to internal organs of the blue crab.

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