Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats

J. Bryan Iorgulescu, Samik P. Patel, Jack Louro, Christian M. Andrade, Andre R. Sanchez, Damien D Pearse

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Schwann cell (SC) transplantation exhibits significant potential for spinal cord injury (SCI) repair and its use as a therapeutic modality has now progressed to clinical trials for subacute and chronic human SCI. Although SC implants provide a receptive environment for axonal regrowth and support functional recovery in a number of experimental SCI models, axonal regeneration is largely limited to local systems and the behavioral improvements are modest without additional combinatory approaches. In the current study we investigated whether the concurrent delivery of the polyamine putrescine, started either 30 min or 1 week after SCI, could enhance the efficacy of SCs when implanted subacutely (1 week after injury) into the contused rat spinal cord. Polyamines are ubiquitous organic cations that play an important role in the regulation of the cell cycle, cell division, cytoskeletal organization, and cell differentiation. We show that the combination of putrescine with SCs provides a significant increase in implant size, an enhancement in axonal (sensory and serotonergic) sparing and/or growth, and improved open field locomotion after SCI, as compared to SC implantation alone. These findings demonstrate that polyamine supplementation can augment the effectiveness of SCs when used as a therapeutic approach for subacute SCI repair.

Original languageEnglish (US)
Article number186385
JournalNeural Plasticity
Volume2015
DOIs
StatePublished - 2015

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Putrescine
Schwann Cells
Spinal Cord Injuries
Axons
Spinal Cord
Polyamines
Growth
Spinal Cord Regeneration
Contusions
Cell Transplantation
Locomotion
Cell Division
Cations
Regeneration
Cell Differentiation
Cell Cycle
Clinical Trials
Wounds and Injuries
Therapeutics

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

Cite this

Acute Putrescine Supplementation with Schwann Cell Implantation Improves Sensory and Serotonergic Axon Growth and Functional Recovery in Spinal Cord Injured Rats. / Iorgulescu, J. Bryan; Patel, Samik P.; Louro, Jack; Andrade, Christian M.; Sanchez, Andre R.; Pearse, Damien D.

In: Neural Plasticity, Vol. 2015, 186385, 2015.

Research output: Contribution to journalArticle

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