Acute melatonin and para-chloroamphetamine interactions on pineal, brain and serum serotonin levels as well as stress hormone levels

Ehrine J P Manzana, Wei Jung A Chen, Thomas Champney

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

para-Chloroamphetamine, an amphetamine analog, alters serotonergic neurochemistry. In previous reports, melatonin (MEL), when administered with other amphetamine analogs, altered the decline in serotonin content produced by these analogs. The present studies assessed the effects of various doses of melatonin and p-chloroamphetamine on serotonin levels in numerous brain regions in male rats. Melatonin (10, 25 or 50 mg/kg, s.c.) and p-chloroamphetamine (3 or 5 mg/kg, s.c.) were administered and, 3 h later, brain samples and serum were collected. Serotonin levels in the serum and various regions of the brain were assayed using high-performance liquid chromatography. Melatonin in combination with a high dose of p-chloroamphetamine (5 mg/kg) produced cumulative deficits in serotonin levels in the serum. However, serotonin levels in the pineal, cortex or brain stem in all combined melatonin and p-chloroamphetamine groups were not significantly different from groups that received p-chloroamphetamine alone. Serum adrenocorticotropin (ACTH) and corticosterone levels were significantly elevated in the melatonin and p-chloroamphetamine combined groups, suggesting that animals receiving both treatments were more stressed than control animals or animals receiving melatonin or p-chloroamphetamine alone. These results indicate that melatonin does not alter p-chloroamphetamine-induced deficits in central serotonin levels. The increased serum adrenocorticotropic hormone, corticosterone and serotonin levels observed following melatonin and p-chloroamphetamine treatment suggest that this combination may have adverse peripheral effects.

Original languageEnglish
Pages (from-to)127-137
Number of pages11
JournalBrain Research
Volume909
Issue number1-2
DOIs
StatePublished - Aug 3 2001
Externally publishedYes

Fingerprint

p-Chloroamphetamine
Melatonin
Serotonin
Hormones
Brain
Serum
Adrenocorticotropic Hormone
Amphetamine
Corticosterone
Neurochemistry
Brain Stem

Keywords

  • Corticosterone
  • Melatonin
  • para-Chloroamphetamine
  • Rat
  • Serotonin
  • Stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Acute melatonin and para-chloroamphetamine interactions on pineal, brain and serum serotonin levels as well as stress hormone levels. / Manzana, Ehrine J P; Chen, Wei Jung A; Champney, Thomas.

In: Brain Research, Vol. 909, No. 1-2, 03.08.2001, p. 127-137.

Research output: Contribution to journalArticle

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