Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition

Kevin Collins, Addys Bode, Robert W. Fernandez, Jessica E. Tanis, Jacob C. Brewer, Matthew S. Creamer, Michael R. Koelle

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Like many behaviors, Caenorhabditis elegans egg laying alternates between inactive and active states. To understand how the underlying neural circuit turns the behavior on and off, we optically recorded circuit activity in behaving animals while manipulating circuit function using mutations, optogenetics, and drugs. In the active state, the circuit shows rhythmic activity phased with the body bends of locomotion. The serotonergic HSN command neurons initiate the active state, but accumulation of unlaid eggs also promotes the active state independent of the HSNs. The cholinergic VC motor neurons slow locomotion during egg-laying muscle contraction and egg release. The uv1 neuroendocrine cells mechanically sense passage of eggs through the vulva and release tyramine to inhibit egg laying, in part via the LGC-55 tyramine-gated Cl- channel on the HSNs. Our results identify discrete signals that entrain or detach the circuit from the locomotion central pattern generator to produce active and inactive states.

Original languageEnglish (US)
Article numbere21126
JournaleLife
Volume5
Issue numberNOVEMBER2016
DOIs
StatePublished - Nov 16 2016

Fingerprint

Ovum
Locomotion
Chemical activation
Feedback
Tyramine
Networks (circuits)
Eggs
Optogenetics
Neurons
Central Pattern Generators
Neuroendocrine Cells
Vulva
Caenorhabditis elegans
Motor Neurons
Muscle Contraction
Cholinergic Agents
Muscle
Animals
Mutation
Inhibition (Psychology)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Collins, K., Bode, A., Fernandez, R. W., Tanis, J. E., Brewer, J. C., Creamer, M. S., & Koelle, M. R. (2016). Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition. eLife, 5(NOVEMBER2016), [e21126]. https://doi.org/10.7554/eLife.21126

Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition. / Collins, Kevin; Bode, Addys; Fernandez, Robert W.; Tanis, Jessica E.; Brewer, Jacob C.; Creamer, Matthew S.; Koelle, Michael R.

In: eLife, Vol. 5, No. NOVEMBER2016, e21126, 16.11.2016.

Research output: Contribution to journalArticle

Collins, K, Bode, A, Fernandez, RW, Tanis, JE, Brewer, JC, Creamer, MS & Koelle, MR 2016, 'Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition', eLife, vol. 5, no. NOVEMBER2016, e21126. https://doi.org/10.7554/eLife.21126
Collins, Kevin ; Bode, Addys ; Fernandez, Robert W. ; Tanis, Jessica E. ; Brewer, Jacob C. ; Creamer, Matthew S. ; Koelle, Michael R. / Activity of the C. elegans egg-laying behavior circuit is controlled by competing activation and feedback inhibition. In: eLife. 2016 ; Vol. 5, No. NOVEMBER2016.
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