Activity of long-chain Acyl-CoA synthetase is required for maintaining meiotic arrest in Xenopus laevis

Hua Weis Wang, Jun Shun Fang, Xia Kuang, Li Yun Miao, Chao Wang, Guo Liang Xia, Mary Lou L King, Jian Zhang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In most vertebrates, fully grown oocytes are arrested in meiotic prophase I and only resume the cell cycle upon external stimuli, such as hormones. The proper arrest and resumption of the meiotic cycle is critical for reproduction. A GalphaS signaling pathway essential for the arrest is conserved in organisms from Xenopus to mouse and human. A previous gene association study implicated that mutations of human ACSL6 may be related to premature ovarian failure. However, functional roles of ACSL6 in human infertility have yet to be reported. In the present study, we found that triacsin C, a potent and specific inhibitor for ACSL, triggers maturation in Xenopus and mouse oocytes in the absence of hormone, suggesting ACSL activity is required for the oocyte arrest. In Xenopus, acsl1b may fulfill a major role in the process, because inhibition of acsl1b by knocking down its RNA results in abnormal acceleration of oocyte maturation. Such abnormally matured eggs cannot support early embryonic development. Moreover, direct inhibition of protein palmitoylation, which lies downstream of ACSLs, also causes oocyte maturation. Furthermore, palmitoylation of Galphas, which is essential for its function, is inhibited when the ACSL activity is blocked by triacsin C in Xenopus. Thus, disruption of ACSL activity causes inhibition of the Galphas signaling pathway in the oocytes, which may result in premature ovarian failure in human.

Original languageEnglish
Article numberArticle 74
JournalBiology of Reproduction
Volume87
Issue number3
DOIs
StatePublished - Sep 1 2012

Fingerprint

Coenzyme A Ligases
Acyl Coenzyme A
Xenopus laevis
Oocytes
Xenopus
Lipoylation
Primary Ovarian Insufficiency
Hormones
Meiotic Prophase I
Infertility
Eggs
Embryonic Development
Reproduction
Vertebrates
Cell Cycle
RNA
Mutation
Genes

Keywords

  • Long-chain acyl-CoA synthetase
  • Meiotic arrest
  • Meiotic maturation
  • Oocyte maturation
  • Palmitoylation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wang, H. W., Fang, J. S., Kuang, X., Miao, L. Y., Wang, C., Xia, G. L., ... Zhang, J. (2012). Activity of long-chain Acyl-CoA synthetase is required for maintaining meiotic arrest in Xenopus laevis. Biology of Reproduction, 87(3), [Article 74]. https://doi.org/10.1095/biolreprod.112.100511

Activity of long-chain Acyl-CoA synthetase is required for maintaining meiotic arrest in Xenopus laevis. / Wang, Hua Weis; Fang, Jun Shun; Kuang, Xia; Miao, Li Yun; Wang, Chao; Xia, Guo Liang; King, Mary Lou L; Zhang, Jian.

In: Biology of Reproduction, Vol. 87, No. 3, Article 74, 01.09.2012.

Research output: Contribution to journalArticle

Wang, HW, Fang, JS, Kuang, X, Miao, LY, Wang, C, Xia, GL, King, MLL & Zhang, J 2012, 'Activity of long-chain Acyl-CoA synthetase is required for maintaining meiotic arrest in Xenopus laevis', Biology of Reproduction, vol. 87, no. 3, Article 74. https://doi.org/10.1095/biolreprod.112.100511
Wang, Hua Weis ; Fang, Jun Shun ; Kuang, Xia ; Miao, Li Yun ; Wang, Chao ; Xia, Guo Liang ; King, Mary Lou L ; Zhang, Jian. / Activity of long-chain Acyl-CoA synthetase is required for maintaining meiotic arrest in Xenopus laevis. In: Biology of Reproduction. 2012 ; Vol. 87, No. 3.
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