Activity of human immunodeficiency virus type 1 promoter/TAR regions and tat1 genes derived from individuals with different rates of disease progression

Frank Kirchhoff, Thomas C. Greenough, Marion Hamacher, John L. Sullivan, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Different rates of disease progression may be associated with different human immunodeficiency virus type 1 (HIV-1) promoter and/or transactivator activities We therefore analyzed the sequences and activities of the first exon of Tat, tat1, and the promoter/trans-acting responsive (TAR) regions amplified directly from peripheral blood mononuclear cells obtained from five long-term nonprogressors and eight progressing HIV-1-infected individuals. The majority of tat1 alleles and promoter/TAR regions from all patients were intact and showed comparable activities in transient reporter assays. A substantial number of point mutations and some length variations were observed in the promoter/TAR region. In a single nonprogressor, the Sp1 binding site 3 was consistently altered and the transcriptional activity in the presence of Tat was diminished. Some LTR clones from a rapid progressor contained a fourth Sp1 binding site, which was associated with an elevated basal promoter activity. These data suggest that defects in the promoter/TAR region or tat1 are rare and that different promoter/transactivator activities are not commonly associated with different progression rates.

Original languageEnglish (US)
Pages (from-to)319-331
Number of pages13
JournalVirology
Volume232
Issue number2
DOIs
StatePublished - Jun 9 1997
Externally publishedYes

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Trans-Activators
Disease Progression
HIV-1
Binding Sites
Point Mutation
Genes
Exons
Blood Cells
Clone Cells
Alleles

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Activity of human immunodeficiency virus type 1 promoter/TAR regions and tat1 genes derived from individuals with different rates of disease progression. / Kirchhoff, Frank; Greenough, Thomas C.; Hamacher, Marion; Sullivan, John L.; Desrosiers, Ronald Charles.

In: Virology, Vol. 232, No. 2, 09.06.1997, p. 319-331.

Research output: Contribution to journalArticle

Kirchhoff, Frank ; Greenough, Thomas C. ; Hamacher, Marion ; Sullivan, John L. ; Desrosiers, Ronald Charles. / Activity of human immunodeficiency virus type 1 promoter/TAR regions and tat1 genes derived from individuals with different rates of disease progression. In: Virology. 1997 ; Vol. 232, No. 2. pp. 319-331.
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