Activities of human DNA polymerase κ in response to the major benzo[a]pyrene DNA adduct

Error-free lesion bypass and extension synthesis from opposite the lesion

Yanbin Zhang, Xiaohua Wu, Dongyu Guo, Olga Rechkoblit, Zhigang Wang

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

In cells, the major benzo[a]pyrene DNA adduct is the highly mutagenic (+)-trans-anti-BPDE-N2-dG. In eukaryotes, little is known about lesion bypass of this DNA adduct during replication. Here, we show that purified human Polκ can effectively bypass a template (+)-trans-anti-BPDE-N2-dG adduct in an error-free manner. Kinetic parameters indicate that Polκ bypass of the (-)-trans-anti-BPDE-N2-dG adduct was ∼41-fold more efficient compared to the (+)-trans-anti-BPDE-N2-dG adduct. Furthermore, we have found another activity of human Polκ in response to the (+)- and (-)-trans-anti-BPDE-N2-dG adducts: extension synthesis from mispaired primer 3′ ends opposite the lesion. In contrast, the two adducts strongly blocked DNA synthesis by the purified human Polβ and the purified catalytic subunits of yeast Polα, Polδ, and Polε right before the lesion. Extension by human Polκ from the primer 3′ G opposite the (+)- and (-)-trans-anti-BPDE-N2-dG adducts was mediated by a -1 deletion mechanism, probably resulting from re-aligning the primer G to pair with the next template C by Polκ prior to DNA synthesis. Thus, sequence contexts 5′ to the lesion strongly affect the fidelity and mechanism of the Polκ-catalyzed extension synthesis. These results support a dual-function model of human Polκ in bypass of BPDE DNA adducts: it may function both as an error-free bypass polymerase alone and an extension synthesis polymerase in combination with another polymerase.

Original languageEnglish
Pages (from-to)559-569
Number of pages11
JournalDNA Repair
Volume1
Issue number7
DOIs
StatePublished - Jul 17 2002
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
Human Activities
DNA Adducts
DNA
Eukaryota
Kinetic parameters
Yeast
benzo(a)pyrene 7,8-diol-9,10-epoxide-N2-deoxyguanosine
benzo(a)pyrene-DNA adduct
Catalytic Domain
Yeasts

Keywords

  • Benzo[a]pyrene
  • DNA adducts
  • Lesion bypass
  • Mutagenesis
  • Polymerase κ
  • Translesion synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Activities of human DNA polymerase κ in response to the major benzo[a]pyrene DNA adduct : Error-free lesion bypass and extension synthesis from opposite the lesion. / Zhang, Yanbin; Wu, Xiaohua; Guo, Dongyu; Rechkoblit, Olga; Wang, Zhigang.

In: DNA Repair, Vol. 1, No. 7, 17.07.2002, p. 559-569.

Research output: Contribution to journalArticle

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N2 - In cells, the major benzo[a]pyrene DNA adduct is the highly mutagenic (+)-trans-anti-BPDE-N2-dG. In eukaryotes, little is known about lesion bypass of this DNA adduct during replication. Here, we show that purified human Polκ can effectively bypass a template (+)-trans-anti-BPDE-N2-dG adduct in an error-free manner. Kinetic parameters indicate that Polκ bypass of the (-)-trans-anti-BPDE-N2-dG adduct was ∼41-fold more efficient compared to the (+)-trans-anti-BPDE-N2-dG adduct. Furthermore, we have found another activity of human Polκ in response to the (+)- and (-)-trans-anti-BPDE-N2-dG adducts: extension synthesis from mispaired primer 3′ ends opposite the lesion. In contrast, the two adducts strongly blocked DNA synthesis by the purified human Polβ and the purified catalytic subunits of yeast Polα, Polδ, and Polε right before the lesion. Extension by human Polκ from the primer 3′ G opposite the (+)- and (-)-trans-anti-BPDE-N2-dG adducts was mediated by a -1 deletion mechanism, probably resulting from re-aligning the primer G to pair with the next template C by Polκ prior to DNA synthesis. Thus, sequence contexts 5′ to the lesion strongly affect the fidelity and mechanism of the Polκ-catalyzed extension synthesis. These results support a dual-function model of human Polκ in bypass of BPDE DNA adducts: it may function both as an error-free bypass polymerase alone and an extension synthesis polymerase in combination with another polymerase.

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