Activation of virus specific CTL clones: Antigen-dependent regulation of interleukin 2 receptor expression

M. E. Andrew, A. M. Churilla, Thomas Malek, V. L. Braciale, T. J. Braciale

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Interleukin 2 (IL 2) is a lymphocyte-specific growth hormone, whose effect on lymphocyte proliferation is exerted through a cell surface receptor expressed on activated lymphocytes. In this report we have used monoclonal antibodies directed to the murine IL 2 receptor to examine the regulation of the IL 2 receptor expression on cloned populations of influenza virus-specific CTL. The CTL clones, which are dependent on both specific antigenic stimulation and exogenous IL 2 for continuous in vitro propagation, express high levels of the IL 2 receptor shortly after antigenic stimulation (day 2 or 3). Over the next 5 to 8 days of in vitro cultivation in IL 2-containing medium, these cloned CTL cells express decreasing levels of IL 2 receptor. Concomitant with this fall in IL 2 receptor expression, the cells become refractory to the IL 2 proliferative stimulus. The cloned cells remain refractory to IL 2 until specifically stimulated by antigen, which induces high levels of the IL 2 receptor on the cells and renders the cells sensitive to IL 2 once again. These results support the concept that IL 2 receptor expression on activated T lymphocytes is transitory and that receptor expression is endogenously regulated in the activated T lymphocytes. These results also suggest that antigen plays a primary role in regulating T lymphocyte proliferation by maintaining IL 2 receptor levels.

Original languageEnglish
Pages (from-to)920-925
Number of pages6
JournalJournal of Immunology
Volume134
Issue number2
StatePublished - Mar 27 1985
Externally publishedYes

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Virus Activation
Interleukin-2 Receptors
Clone Cells
Interleukin-2
Antigens
Lymphocytes
T-Lymphocytes
Cell Surface Receptors
Orthomyxoviridae
Growth Hormone
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Andrew, M. E., Churilla, A. M., Malek, T., Braciale, V. L., & Braciale, T. J. (1985). Activation of virus specific CTL clones: Antigen-dependent regulation of interleukin 2 receptor expression. Journal of Immunology, 134(2), 920-925.

Activation of virus specific CTL clones : Antigen-dependent regulation of interleukin 2 receptor expression. / Andrew, M. E.; Churilla, A. M.; Malek, Thomas; Braciale, V. L.; Braciale, T. J.

In: Journal of Immunology, Vol. 134, No. 2, 27.03.1985, p. 920-925.

Research output: Contribution to journalArticle

Andrew, ME, Churilla, AM, Malek, T, Braciale, VL & Braciale, TJ 1985, 'Activation of virus specific CTL clones: Antigen-dependent regulation of interleukin 2 receptor expression', Journal of Immunology, vol. 134, no. 2, pp. 920-925.
Andrew, M. E. ; Churilla, A. M. ; Malek, Thomas ; Braciale, V. L. ; Braciale, T. J. / Activation of virus specific CTL clones : Antigen-dependent regulation of interleukin 2 receptor expression. In: Journal of Immunology. 1985 ; Vol. 134, No. 2. pp. 920-925.
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