Activation of the GM-CSF promoter by HTLV-I and -II tax proteins

Stephen D Nimer, J. C. Gasson, K. Hu, I. Smalberg, J. L. Williams, I. S Y Chen, Joseph D Rosenblatt

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Production of granulocyte-macrophage colony-stimulating factor (GM-CSF) by normal T lymphocytes requires activation by antigen, mitogen or lectin, whereas T-cell lines transformed by human T-cell leukemia virus type I (HTLV-I) or type II (HTLV-II) constitutively produce high levels of GM-CSF. Using transient co-transfection assays, we demonstrate that introduction of the tax gene of either HTLV-I or HTLV-II is sufficient to activate GM-CSF promoter constructs in an unstimulated T-cell line. The GM-CSF 5' flanking sequences previously shown to be sufficient for GM-CSF induction following T-cell activation are also sufficient for activation by the HTLV tax proteins. The sequences required for trans-activation of GM-CSF are distinct from those required for the activation of other T-cell-inducible genes (IL-2Rα, IL-2) by tax, suggesting that tax can have pleiotropic effects on gene expression in T cells. Constitutive GM-CSF production by HTLV-infected T cells may therefore be due to trans-activation of its promoter by tax. Expression of GM-CSF by HTLV-I infected lymphocytes may be important in the granulocytosis and eosinophilia frequently seen in patients with HTLV-I-induced adult T-cell leukemia/lymphoma.

Original languageEnglish
Pages (from-to)671-676
Number of pages6
JournalOncogene
Volume4
Issue number6
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Human T-lymphotropic virus 2
Granulocyte-Macrophage Colony-Stimulating Factor
Human T-lymphotropic virus 1
T-Lymphocytes
pX Genes
tax Gene Products
Cell Line
Adult T Cell Leukemia Lymphoma
Human T-lymphotrophic virus 1 tax protein
5' Flanking Region
Eosinophilia
Lymphocyte Activation
Mitogens
Lectins
Interleukin-2
Transfection
Lymphocytes
Gene Expression
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Nimer, S. D., Gasson, J. C., Hu, K., Smalberg, I., Williams, J. L., Chen, I. S. Y., & Rosenblatt, J. D. (1989). Activation of the GM-CSF promoter by HTLV-I and -II tax proteins. Oncogene, 4(6), 671-676.

Activation of the GM-CSF promoter by HTLV-I and -II tax proteins. / Nimer, Stephen D; Gasson, J. C.; Hu, K.; Smalberg, I.; Williams, J. L.; Chen, I. S Y; Rosenblatt, Joseph D.

In: Oncogene, Vol. 4, No. 6, 01.01.1989, p. 671-676.

Research output: Contribution to journalArticle

Nimer, SD, Gasson, JC, Hu, K, Smalberg, I, Williams, JL, Chen, ISY & Rosenblatt, JD 1989, 'Activation of the GM-CSF promoter by HTLV-I and -II tax proteins', Oncogene, vol. 4, no. 6, pp. 671-676.
Nimer SD, Gasson JC, Hu K, Smalberg I, Williams JL, Chen ISY et al. Activation of the GM-CSF promoter by HTLV-I and -II tax proteins. Oncogene. 1989 Jan 1;4(6):671-676.
Nimer, Stephen D ; Gasson, J. C. ; Hu, K. ; Smalberg, I. ; Williams, J. L. ; Chen, I. S Y ; Rosenblatt, Joseph D. / Activation of the GM-CSF promoter by HTLV-I and -II tax proteins. In: Oncogene. 1989 ; Vol. 4, No. 6. pp. 671-676.
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