Activation of the estrogen receptor contributes to the progression of pulmonary lymphangioleiomyomatosis via matrix metalloproteinase-induced cell invasiveness

Marilyn K Glassberg Csete, Sharon Elliot, Jason Fritz, Paola Catanuto, Mylene Potier, Roger Donahue, William Stetler-Stevenson, Michael Karl

Research output: Contribution to journalArticle

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Abstract

Context: The role of estrogens in the pathogenesis of lymphangioleiomyomatosis (LAM), an aggressive and destructive, eventually fatal lung disease of women, is poorly understood. Objective: The study was conducted to test the hypothesis that the lung disease in LAM is estrogen mediated and to determine whether estrogens contribute to the invasiveness of LAM. Design: In vitro cell culture of spindle-shaped LAM cells (LAMD-SM) were isolated and propagated from affected lungs. Estrogen receptor (ER)-α and ERβ analyses were conducted by RT-PCR. ERα and ERβ, tissue inhibitor of metalloproteinase-2, and matrix metalloproteinases (MMP)-2 had Western blot analysis for protein assessment. Activity assays were performed for MT1-MMP, MMP-2, and tissue inhibitor of metalloproteinase-2. Assessment of MMP-2 promoter function was done via transfection assays. Cell invasion chambers were used to determine and quantitate cell invasiveness. Setting: The study was conducted at an academic medical center. Patients: Tissue and cells were obtained from patients as outlined in approved institution review board protocol (97/007). Intervention: LAMD-SM cells were treated with a specific MMP-2 antibody or a nonspecific inhibitor, doxycycline. Main Outcome Measures: Activity of MMP-2 and invasiveness of LAMD-SM cells were measured. Results: LAMD-SM cells express functional ERs (ERα and ERβ), which undergo rapid intracellular turn-over in their unbound state. 17β-Estradiol (E2) enhances the transcriptional ER activity. E2-induced ER activation increases synthesis and activity of MMP-2 through posttranscriptional mechanisms in LAMD-SM. The E2/ER-mediated increase of MMP-2 promotes LAMD-SM invasiveness, in assays in vitro, which can be inhibited by specific antibodies against MMP-2 or doxycycline, an inhibitor of MMPs. Conclusion: The invasion and destruction of lung parenchyma in LAM is, at least partially, an estrogen-MMP-driven process, which has major implications for therapeutic interventions.

Original languageEnglish
Pages (from-to)1625-1633
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume93
Issue number5
DOIs
StatePublished - May 1 2008

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Lymphangioleiomyomatosis
Matrix Metalloproteinases
Matrix Metalloproteinase 2
Estrogen Receptors
Chemical activation
Lung
Estrogens
Tissue Inhibitor of Metalloproteinase-2
Assays
Pulmonary diseases
Matrix Metalloproteinase Inhibitors
Doxycycline
Lung Diseases
Thermodynamic properties
Matrix Metalloproteinase 14
Antibodies
Cell culture
Transfection
Estradiol
Cell Culture Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Activation of the estrogen receptor contributes to the progression of pulmonary lymphangioleiomyomatosis via matrix metalloproteinase-induced cell invasiveness. / Glassberg Csete, Marilyn K; Elliot, Sharon; Fritz, Jason; Catanuto, Paola; Potier, Mylene; Donahue, Roger; Stetler-Stevenson, William; Karl, Michael.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 93, No. 5, 01.05.2008, p. 1625-1633.

Research output: Contribution to journalArticle

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abstract = "Context: The role of estrogens in the pathogenesis of lymphangioleiomyomatosis (LAM), an aggressive and destructive, eventually fatal lung disease of women, is poorly understood. Objective: The study was conducted to test the hypothesis that the lung disease in LAM is estrogen mediated and to determine whether estrogens contribute to the invasiveness of LAM. Design: In vitro cell culture of spindle-shaped LAM cells (LAMD-SM) were isolated and propagated from affected lungs. Estrogen receptor (ER)-α and ERβ analyses were conducted by RT-PCR. ERα and ERβ, tissue inhibitor of metalloproteinase-2, and matrix metalloproteinases (MMP)-2 had Western blot analysis for protein assessment. Activity assays were performed for MT1-MMP, MMP-2, and tissue inhibitor of metalloproteinase-2. Assessment of MMP-2 promoter function was done via transfection assays. Cell invasion chambers were used to determine and quantitate cell invasiveness. Setting: The study was conducted at an academic medical center. Patients: Tissue and cells were obtained from patients as outlined in approved institution review board protocol (97/007). Intervention: LAMD-SM cells were treated with a specific MMP-2 antibody or a nonspecific inhibitor, doxycycline. Main Outcome Measures: Activity of MMP-2 and invasiveness of LAMD-SM cells were measured. Results: LAMD-SM cells express functional ERs (ERα and ERβ), which undergo rapid intracellular turn-over in their unbound state. 17β-Estradiol (E2) enhances the transcriptional ER activity. E2-induced ER activation increases synthesis and activity of MMP-2 through posttranscriptional mechanisms in LAMD-SM. The E2/ER-mediated increase of MMP-2 promotes LAMD-SM invasiveness, in assays in vitro, which can be inhibited by specific antibodies against MMP-2 or doxycycline, an inhibitor of MMPs. Conclusion: The invasion and destruction of lung parenchyma in LAM is, at least partially, an estrogen-MMP-driven process, which has major implications for therapeutic interventions.",
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AU - Glassberg Csete, Marilyn K

AU - Elliot, Sharon

AU - Fritz, Jason

AU - Catanuto, Paola

AU - Potier, Mylene

AU - Donahue, Roger

AU - Stetler-Stevenson, William

AU - Karl, Michael

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